同济大学学报（医学版）2018，Vol.39Issue(1)：23-28,6.DOI:10.16118/j.1008-0392.2018.01.006
Prominin1在C57BL/6小鼠视网膜色素上皮中的表达
Expression of Prominin1 in retinal pigment epithelium of C57BL/6 mice at different development stages
摘要
Abstract
Objective To investigate the expression of Prominin1 in Retinal Pigment Epithelium (RPE) of mice at differential development stages.Methods Tamoxifen was intraperitoneally injected in pregnant or postnatal cre/ERT2+/CAG-ZsGreen1 + transgenic mice.The expression of Prominin1 in RPE of transgenic mice was examined by confocal laser scanning microscope with frozen section.In addition,the expression of Prominin 1 in developmental RPE of wild type mice was detected with realtime PCR and immunofluorescence.Results Prominin1was expressed in RPE of E13.5,E15.5 of transgenic mice tracer,however,not expressed in postnatal mice (P1).Real-time PCR showed that the expression of prominin1 was significantly higher in embryonic stages (E10.5,E13.5,E15.5 and E17.5) than that in postnatal stages (P＜0.05).Moreover,the expression of Prominin1 in RPE at E13.5 and E15.5 was significantly higher than that in other embryonic periods(P＜0.05 or P＜0.01).Immunofluorescence showed that Prominin1 was expressed at E10.5,E13.5,E15.5 and E17.5,but not expressed at P1.Conclusion Prominin 1 is expressed in RPE at different embryonic stages of mice,especially,at E13.5 and E15.5.关键词
视网膜色素上皮/Prominin1/转基因小鼠Key words
retinal pigment epithelium/Prominin1/transgenic mice分类
医药卫生引用本文复制引用
邵志华,李瑛泽,许洁,于金军,刘志学,李思光..Prominin1在C57BL/6小鼠视网膜色素上皮中的表达[J].同济大学学报（医学版）,2018,39(1):23-28,6.基金项目
国家自然科学基金(31571405) （31571405）
