同济大学学报（医学版）2018，Vol.39Issue(1)：34-40,7.DOI:10.16118/j.1008-0392.2018.01.008
外源性FGF-2对高糖环境下内皮祖细胞功能的影响
Exogenous FGF-2 improves biological activity of endothelial progenitor cells damaged by high glucose
摘要
Abstract
Objective To investigate the effects of exogenous basic fibroblast growth factor-2 (FGF-2) on the damaged biological activity of endothelial progenitor cells (EPCs) induced by high glucose.Methods Bone marrow endothelial progenitor cells from C57BL/6 mice was isolated,then cultured in vitro and identified by flow cytometry and immunofluorescence staining.EPCs were treated with high glucose (30 mmol/L) and high glucose + FGF-2 (30,50,100,150 and 300 ng/mL) respectively,and normal EPCs were taken as a blank control.The cell apoptosis was detected by TUNEL,the migration and tube formation ability of EPCs in vitro were observed by Transwell chamber and Matrigel reagent;the expression of activated β-catenin and total β-catenin was detected by Western blot.Mouse hind limb ischemia model was constructed;and the ischemic hind limb mice were injected with EPCs obtained from blank,normal high glucose and high glucose + FGF2 (150 ng/mL) groups,respectively.The blood flow recovery was observed by laser Doppler flowmetry in mice with ischemic hind limbs.Results EPCs in high glucose group were significantly higher than those in blank control group (P＜0.01),and migration and tube formation ability significantly decreased (P＜0.01).Compared with high glucose group,cell apoptosis significantly decreased and the migration and tube formation ability significantly increased in the high glucose + FGF-2 group;and the effect of 150 ng/mL FGF-2 was the strongest (P＜0.05,P＜0.01).Western blotting analysis showed that the ratio of activated β-catenin/total β-catenin in the high glucose group was significantly lower than that in the control group,while the ratio in high glucose + FGF-2 groups was significantly higher than that in the high glucose group.In vivo experiments showed that the blood flow recovery of the ischemic hind limbs of mice in the high glucose group was significantly lower than that in the normal group,while the blood flow recovery in high glucose + FGF-2 group was significantly higher than that in the high glucose group.Conclusion Exogenous FGF-2 can repair the damaged function of EPCs induced by high glucose,which may be associated with activating Wnt/β-catenin signaling pathway.关键词
碱性成纤维细胞生长因子/内皮祖细胞/血管新生/Wnt/β-catenin信号通路/高糖环境Key words
fibroblastgrowth factor-2/endothelial progenitor cell/angiogenesis/Wnt/β-catenin signaling pathway/high glucose environment分类
医药卫生引用本文复制引用
李阳,陆晨晖,陆骊工,张海军,滕皋军,李茂全..外源性FGF-2对高糖环境下内皮祖细胞功能的影响[J].同济大学学报（医学版）,2018,39(1):34-40,7.基金项目
国家自然科学基金(8157070468) （8157070468）
