同济大学学报（医学版）2018，Vol.39Issue(1)：47-53,7.DOI:10.16118/j.1008-0392.2018.01.010
敲降TMEM45A基因对卵巢癌细胞增殖、黏附及侵袭的影响
Knockdown of TMEM45A gene suppresses proliferation, induces cell cycle arrest and reduces invasion ability of human ovarian cancer cells
摘要
Abstract
Objective To investigate the effect of TMEME45A gene on the proliferation and invasion of ovarian cancer cells and related signaling pathway.Methods The expression of TMEM45A was compared between ovarian cancer and normal tissues based on the RNA-sequencing data of the ovarian cancer cohort from The Cancer Genome Atlas (TCGA) project.The expression of TMEM45A was also detected by RT-PCR in 25 pairs of ovarian cancer tissues and the matched non-cancerous tissues.The expression of TMEM45A in ovarian cancer HO-8910 and A2780 cells was down-regulated by RNA interference.Cell proliferation,cell cycle distribution,cell adhesion and invasion ability were detected by Cell Counting Kit-8 assay (CCK-8),propidium iodide (PI) staining,cell adhesion and Transwell assays,respectively.In addition,the mRNA and protein expression levels of transforming growth factor-β (TGF-β1 and TGF-β2),Ras homolog family member A (RhoA) and Rho-associated kinase 2 (ROCK2) were detected by RT-PCR and Western blotting,respectively.Results TMEM45A was over-expressed in ovarian cancer(P＜0.001).Silence of TMEM45A suppressed cell proliferation (P＜0.05)and increased cell population in the G1 phase significantly (P ＜ 0.05).Knock-down of TMEM45A inhibited cell adhesion and invasion (P＜0.05),down-regulated the expression of TGF-β1/2,RhoA and ROCK2(P＜0.05).Conclusion TMEM45A may work as a oncogene of ovarian cancer,knockdown of TMEM45A gene may be a potential therapeutic strategy for ovarian cancer.关键词
跨膜蛋白45A/卵巢癌/ras同源家族蛋白A/ras同源家族蛋白相关激酶2Key words
transmembrane protein 45A/ovarian cancer/RhoA/ROCK2分类
医药卫生引用本文复制引用
门传娣,任青,郭静,罗宁,杨伟红,程忠平..敲降TMEM45A基因对卵巢癌细胞增殖、黏附及侵袭的影响[J].同济大学学报（医学版）,2018,39(1):47-53,7.基金项目
上海市医学重点专科建设计划(2A2015A33) （2A2015A33）
