生殖医学杂志2018，Vol.27Issue(4)：316-321,6.DOI:10.3969/j.issn.1004-3845.2018.04.005
维生素D对复发性流产患者外周血自然杀伤细胞的调节作用
Modulatory effects of vitamin D on peripheral NK cells in patients with recurrent miscarriage
摘要
Abstract
Objective:To investigate the modulatory effects of vitarnin D(VD)on peripheral blood natural killer (NK)cells in patients with recurrent miscarriage(RM).Methods:The Peripheral bloods of mid-luteal phase were collected from 99 patients with RM.The quantity and cytotoxicity of NK cells in peripheral blood were detected by flow cytometry.The RM patients were divided into three groups according to the levels of vitamin D:VD normal group(VDN),VD insufficiency group(VDI),and VD deficiency group(VDD).Meanwhile,1,25 (OH) 2D was supplemented to the RM patients with VD deficiency to observe the regulatory effect of VD supplementation on NK cells.Results:There was no significant difference in the percentage of CD3-CD56-NK cells among the three groups(P＞0.05).The effector to target cell(E∶T)ratio of NK cytotoxicity was 50.∶ 1(51.6±8.8 vs.45.3±12.4,P＜0.05),25:1(43.2±9.1 vs.35.8±11.8,P＜0.01)and 12.5:1(30.2±10.3 vs.23.0±10.6,P＜0.01)in the VD insufficiency group(VDI) were significantly higher than that in the VD normal group(VDN).After 1,25(OH)2D supplementation,no significant difference in the percentage of CD3-CD56-NK cells was observed among the three groups.However,the effector to target cell(E∶T) ratioof NK cytotoxicitywas 50 ∶ 1(41.6±14.0 vs.51.0±10.5,P＜0.01),25 ∶ 1(34.9±14.3 vs.43.1±10.8,P＜0.01),12.5 ∶ 1(22.6±11.4 vs.29.9±10.7,P＜0.01),which was significantly reduced with 1,25(OH)2D supplementation.Conclusions:NK cytotoxicity is increased in peripheral blood of RM patients with low-level vitamin D.Supplementing of 1,25(OH)2D can reduce NK cytotoxicity to a certain extent.关键词
维生素D/NK细胞比例/NK细胞毒性/复发性流产Key words
Vitamin D/NK cell percentage/NK cytotoxicity/Recurrent miscarriage引用本文复制引用
徐士儒,王云霞,连若纯,张諝,陈伟洪,何来宾,陈婉如,陈现..维生素D对复发性流产患者外周血自然杀伤细胞的调节作用[J].生殖医学杂志,2018,27(4):316-321,6.基金项目
深圳市基础研究基金(JCYJ20160427113303899) （JCYJ20160427113303899）
中华医学会临床医学科研专项资金生殖医学青年医师研究与发展项目(17020340703) （17020340703）
