中国组织工程研究2017，Vol.21Issue(36)：5818-5823,6.DOI:10.3969/j.issn.2095-4344.2017.36.014
猕猴急性脊髓全横断损伤血清的代谢组学分析
Serum metabolomic profiling of acute complete spinal cord transection in macaques
摘要
Abstract
BACKGROUND:Non-targeted metabolomic profiling is used to uncover metabolic changes and to identify relationships between metabolites and spinal cord injury,which contributes to further understanding the pathophysiological process and mechanisms of secondary spinal cord injury.OBJECTIVE:To detect and analyze the serum metabolite changes after complete spinal cord transaction in macaques,explore its relationship with the pathophysiological progress of spinal cord injury,and screen the potential biomarkers.METHODS:Five adult macaques were selected,in which the models of complete spinal cord transaction were established.The serum metabolic features were detected using a non-targeted metabolic profiling strategy based on gas chromatography time-of-flight mass spectrometry at 1 day before modeling,3 hours (superacute phase) and 3 days (acute phase) after modeling.After compared with the spectrometry profiling,recognizing the metabolites,searching for differential metabolites and the related metabolic pathways,the pathophysiological process and mechanisms were analyzed.RESULTS AND CONCLUSION:Three hundred and fifty-eight chromatographic peaks were obtained for subsequent data analysis.Fourteen metabolites,including low-molecular-weight organic acid,amino acids,fatty acid and carbohydrate,were identified as differential metabolites.To conclude,the acute phase of complete spinal cord transection is closely related to some metabolic pathways,such as amino acid metabolism,tricarboxylic acid cycle and pyruvate metabolism.关键词
组织构建/组织工程/猕猴,脊髓损伤,代谢组学,气相飞行时间质谱,三羧酸循环,氨基酸代谢,丙酮酸代谢,主成分分析/国家自然科学基金分类
医药卫生引用本文复制引用
唐涛,程建华,杨树广,高健,黄涛,王滨,董芳霆,刘少君..猕猴急性脊髓全横断损伤血清的代谢组学分析[J].中国组织工程研究,2017,21(36):5818-5823,6.基金项目
国家自然科学基金青年-面上连续资助项目(81370051) （81370051）
the General Project of National Natural Science Youth Foundation,No.81370051 （）
