中国康复理论与实践2018，Vol.24Issue(4)：385-390,6.DOI:10.3969/j.issn.1006-9771.2018.04.003
血清对大鼠星形胶质细胞形态和水通道蛋白-4表达的影响
Effect of Serum on Cultured Astrocytes Morphology and Expression of Aquaporin-4 in Rats
摘要
Abstract
Objective To explore the effect of blood-brain barrier disruption on expression of AQP-4,through comparing the cell morphology and the expression of aquaporin-4(AQP-4)of cultured astrocytes in medium with and without fetal bovine serum(FBS). Methods Cerebral cortical astrocytes from female Wistar rats were cultured in serum free medium,DMEM supplement-ed with 10% FBS,and serum free medium supplemented with 10% FBS.Phase contrast microscope was used to detect the cell morphology and cell size. Immunofluorescence staining and reverse real-time quantitative poly-merase chain reaction(RT-qPCR)were used to examine the expression of glial fibrillary acidic protein(GFAP), AQP-4 and metabotropic glutamate receptor 5(mGluR5). Results Astrocytes in serum free medium showed extensive process bearing morphology,small body and nucleus,and high refractivity.In contrast,in two kinds of 10% FBS-containing medium,astrocytes were flat with large body and nucleus,weak refractivity,as well as short process.Analysis of immunofluorescence staining and RT-qPCR revealed a down-regulation of GFAP and AQP-4 protein and mRNA expression in two kinds of 10% FBS-con-taining medium, compared with that in serum free medium (P<0.001), however, there was no difference in mGluR5 protein and mRNA expression(P>0.05). Conclusion FBS changed astrocyte morphology and down-regulated the expression of GFAP and AQP-4.关键词
星形胶质细胞/血清/水通道蛋白-4/胶质纤维酸性蛋白/代谢型谷氨酸受体5/大鼠Key words
astrocytes/serum/aquaporin-4/glial fibrillary acidic protein/metabotropic glutamate receptor subtype 5/rats分类
医药卫生引用本文复制引用
贾梅,师忠芳,王玉娇,闫旭,徐立新,董丽萍,李佳欣,陈烨,袁芳..血清对大鼠星形胶质细胞形态和水通道蛋白-4表达的影响[J].中国康复理论与实践,2018,24(4):385-390,6.基金项目
1.国家自然科学基金项目(No.81271286) （No.81271286）
2.北京市自然科学基金项目(No.7152027) （No.7152027）
3.北京市神经外科研究所创新探索基金项目(No.2017-5)Supported by National Natural Science Foundation of China(No.81271286),Beijing Natural Science Foundation(No.7152027)and Beijing Neurosurgical Institute Project(No.2017-5) （No.2017-5）
