摘要
Abstract
Objective To explore the effect of rosuvastatin on the myocardial apoptosis in rats with acute myocardial infarction (AMI) and its influence on the expression of caspase-3 gene.Methods Forty-five healthy male SD rats,10 of them were sham operation group only ligated with the left anterior descending coronary artery without ligation),other 35 rats were construct the AMI model,the 27 survival model rats were randomly divided into test group (n =14) and model group (n =13).Test group was gavaged 1 mg · kg-1 · d-1 rosuvastatin from the 1st day post-operation.Model group was gavaged equivalent 0.9％ NaC1 from the 1 st day post-operation.Each group was intervened for 1 month continuously.The myocardial apoptosis and myocardial infarction of rats in each group were observed by terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL) method,and the caspase-3 gene was compared by immunohistochemistry.Results The TUNEL detection results showed that in model group,the myocardial cell was schistose sepia staining,and the sepia staining decreased significantly in test group,and the sham-operation group showed a scattered and bit sepia staining.The apoptotic index (AI) of sham-operation group,test group and model group were 2.33 ± 1.10,6.01 ±3.12,17.13 ±8.76.Compared with sham-operation group,the AI in test group and model group were significantly increased (P ＜0.05),while the test group decreased significantly compared with model group (P ＜ 0.01).The immunohistochemistry detection results showed that,there was a little brown staining in sham-operation group,and the brown staining parts increased significantly,and the staining power aggravated significantly in test group and model group,but the brown staining parts and staining power in test group were not as good as model group.Compared with sham-operation group (94.98 ± 13.44),the caspase-3 positive staining index of test group (110.67 ± 18.59) and model group (138.25 ±20.93) were significantly increased (P ＜0.05),but the caspase-3 of test group decreased significantly compared with model group (P ＜0.01).Conclusion Rosuvastatin can restrain the myocardial apoptosis of AMI model rats,and the action mechanism may be related to the downregulation of the expression of apoptosis related adjustment gene caspase-3 in cardiac muscle tissue.关键词
急性心肌梗死/瑞舒伐他汀/心肌细胞/凋亡/胱天蛋白酶-3Key words
acute myocardial infarction/rosuvastatin/myocardial cell/apoptosis/caspase-3分类
医药卫生
