Abstract
Objective To investigate the effect of Compound Matrine Injection(CMI) on the levels of nuclcar-associated antigen Ki-67,survivin,serum estradiol (E2),P53 tumor suppressor gene and programmed cell death 4 (PDCD4) in model rats of breast cancer.Methods Ninety male SD rats were selected,of them 15 rats were randomly selected as normal group.The rest of the 75 rats were used for breast cancer model construction by methyl-nitroso-urea intraperitoneal injection.Model rats that successfully established were randomly divided into five groups,15 rats in each group:control group (tamoxifen mixture 40 mL· kg-1 by gavage),the model group,high,middle and low dose experimental groups (20,40,60 mL · kg-1 CMI by intraperitoneal injection,respectively).After 9 weeks of drug interference,the serum level of E2 was determined by radioimmunoassay.The levels of Ki-67,P53,phosphorylation AKT and PDCD4,survivin in serum were determined by ELISA.Results After administration,levels of E2 (ng · L-1) were 37.54 ±5.54,69.52 ±8.58,65.33 ±7.21,41.08 ±5.87,42.94 ±6.06 and 49.27 ±6.73 in normal,model,three dose experimental and control groups,respectively.The levels of survivin (ng · L-1) in each groups were 6.13 ±0.84,62.18 ±8.42,29.68 ±3.39,16.76 ±2.73,16.17 ± 1.27 and 59.85 ±7.48,respectively.The levels of Ki-67 (ng · L-1) in above six groups were 16.20±2.14,71.28 ± 10.87,68.88 ± 8.62,21.54 ± 3.17,23.75 ±3.17 and 38.63 ±5.72,respectively.The levels of P53 (ng · L-1) in above six groups were 10.86 ± 1.13,49.17 ± 6.48,45.13 ± 5.17,12.18 ± 1.75,13.17 ± 1.36 and 27.44 ± 3.52,respectively;phosphorylation AKT (ng· L-1) in above six groups were 17.12 ±4.12,37.53 ±5.71,23.21 ± 1.86,21.56 ±5.21,19.32 ±3.21,23.21 ±1.86,respectively.The levels of serum PDCD4 (ng · L-1) in above six groups were 22.85 ± 0.31,7.72 ± 1.18,8.83 ± 1.18,19.29 ± 2.74,20.06 ± 2.25 and 14.83 ± 6.28,respectively;compared with model group,the levels of the indecators in normal,model,high,middle dose experimental and control groups had significantly(all P ＜ 0.05);compared with control group,high and middle dose experimental groups significantly (all P ＜ 0.05).Conclusion Middle and high dose of CMI could obviously decrease the levels of Ki-67,surviving,E2,P53 and PD-CD4 in the model rats of breast cancer.关键词
复方苦参注射液/乳腺癌/增殖核抗原Ki-67/生存素/抑癌基因P53/程序细胞死亡因子4Key words
Compound Matrine Injection/breast cancer/proliferating cell nuclear antigen Ki-67/survivin/P53 tumor suppressor gene/programmed cell death 4分类
医药卫生
