中国临床药理学杂志2018，Vol.34Issue(2)：145-147,3.DOI:10.13699/j.cnki.1001-6821.2018.02.015
三氧化二砷联合多西他赛对人卵巢癌细胞株HO8910的抗肿瘤作用机制研究
Study of mechanisms of anti-tumor aganist arsenic trioxide combined with docetaxel for human ovary cancer cell line HO8910
摘要
Abstract
Objective To investigate the effect of arsenic trioxide (As2O3) and docetaxel on cell proliferation and apoptosis of ovarian cancer cell line HO8910 in vitro.Methods The ovarian cancer cells were divided into blank group (given 0.9％ NaCl),model group (8 μmol · L-1As2O3),control group (1 mg · L-1 docetaxel) and experimental group(8 μmol · L-1 As2O3 + 1 mg· L-1 docetaxel).The 4 groups were cultured for 72 h.The inhibitory rate of drugs on cell growth was detected by MTT.The expression of PKR-like ER kinase (PERK),C/EBP homologous protein(CHOP),Caspase 3 and B-cell lymphoma-2(Bcl-2)were detected by Western blot.Results After the intervention of drugs for 72 h,the inhibition rates of ovarian cancer cells in model group,control group and experimental group were (3.04 ± 0.76)％,(14.91 ± 1.52) ％,(38.79 ± 2.94) ％.Compared with model group,the differences in experimental group and control group were statistically significant (all P ＜ 0.01);compared with control group,the differences in experimental group were statistically significant (P ＜ 0.01).After 72 h of drug intervention,the PERK in model group,control group and experimental group were 6.27 ±0.18,6.28 ±0.19,7.74 ±0.90;the CHOP in above three groups were 6.25 ±0.24,6.27 ±0.16,7.51 ±0.49;the Caspase in above three groups were 37.14 ±0.21,37.21 ±0.63,9.40 ±0.33.Compared with model group,the differences in experimental group were statistically significant (all P ＜ 0.05);compared with control group,the differences in experimental group were statistically significant (all P ＜ 0.01).Conclusion Arsenic trioxide combination with docetaxel can activate ER stress apoptosis channel on ovarian cancer cell lines,supress ovarian cancer cell proliferation and induce apoptosis in ovarian cancer cells.关键词
三氧化二砷/多西他赛/卵巢癌细胞HO8910/凋亡蛋白/内质网Key words
arsenic trioxide/docetaxel/ovarian cancer cell HO8910/apoptotic protein/endoplasmic reticulum分类
医药卫生引用本文复制引用
车力凡,王颖,刘瑶..三氧化二砷联合多西他赛对人卵巢癌细胞株HO8910的抗肿瘤作用机制研究[J].中国临床药理学杂志,2018,34(2):145-147,3.基金项目
潍坊市卫生局科研项目计划基金资助项目(2010017) （2010017）
