中国农业科学2018，Vol.51Issue(5)：952-963,12.DOI:10.3864/j.issn.0578-1752.2018.05.013
根施褪黑素对NaCl胁迫下葡萄内源褪黑素及叶绿素荧光特性的影响
Effect of Root-Applied Melatonin on Endogenous Melatonin and Chlorophyll Fluorescence Characteristics in Grapevine Under NaCl Stress
摘要
Abstract
[Objective] The content of the endogenous MT,the key metabolites,and the effect ofphotoinhibition on the leaf were studied in grapevine under NaC1 stress,and the results will provide a reference for the application of MT in the future.[Method] One-year old potted'Vidal Blanc'grapevines (Vitis vinifera cv.Vidal Blanc) were used as materials.The grapevines were irrigated with NaCl (100 nmol·L-1) and NaCl+MT (100 nmol·L-1),and then the content and distribution of the endogenous MT,MT precursors serotonin (5-hydroxytryptamine,5-HT),and then the main metabolites of melatonin 2-hydroxymelatonin (2-OHMel) in different organs of different treatment plants,and the chlorophyll fluorescence characteristics in leaves and the metabolism of endogenous MT were measured and analyzed.[Result] The results showed that there were obvious temporal and spatial changes in the content of endogenous MT,5-HT and 2-OHMel in different organs of plants under NaCl stress,and the MT content of roots reached the peak value after 7 days treatment,followed by the shoots,and the content of leaves were the lowest.With the prolongation of the stress time,the MT content in the roots decreased significantly,while the 5-HT content in the shoots increased.Compared with the only NaCl stress,the 5-HT content in the leaves,the MT content in the roots and the contents of 2-OHMel in the shoots and leaves increased significantly after root-applied MT.The shape of OJIP curve was significantly changed,Fm and leaf maximum photochemical efficiency (Fv/Fm) significantly decreased by 31.6％ and 11.6％,the non-photochemical quenching (NPQ) was significantly increased,and the linear electron transport rate of PSⅠ (ETR(Ⅰ)) and PSⅡ (ETR(Ⅱ)) were significantly decreased under NaC1 stress.While the Fm and Fv/Fm in leaves were reduced by 12.9％ and 7.3％,the increase of K and J points in the OJIP curve was significantly reduced by 23.6％ and 11.3％,and the utilization efficiency of the light linear and electron transfer rate is significantly improved under NaC1 stress and MT treatment.[Conclusion] NaC1 stress induced the inhibition of photosystem activity in grapevine leaves,but promoted the synthesis of MT in the plant.Photoinhibition was alleviated under MT treatment,root-applied MT promoted the metabolism and distribution of MT in various organs of plants,alleviated the damaged degree of photosynthesis of grapevine leaves by NaCl stress.关键词
NaCl胁迫/葡萄/叶绿素荧光/褪黑素/5-羟色胺/2-羟基褪黑素Key words
NaCl stress/grapevine/chlorophyll fluorescence/melatonin/5-hydroxytryptamine/2-hydroxymelatonin引用本文复制引用
卞凤娥,肖秋红,郝桂梅,孙永江,陆文利,杜远鹏,翟衡..根施褪黑素对NaCl胁迫下葡萄内源褪黑素及叶绿素荧光特性的影响[J].中国农业科学,2018,51(5):952-963,12.基金项目
国家现代葡萄产业技术体系建设专项(CARS-29)、长江学者和创新团队发展计划(IRT15R42) （CARS-29）
