中国药房2018，Vol.29Issue(8)：1027-1031,5.DOI:10.6039/j.issn.1001-0408.2018.08.04
肿瘤标志物PHPAA分子印迹聚合物的合成与性能研究
Study on Synthesis of Tumor Marker PHPAA Molecular Imprinted Polymers and Its Performance
摘要
Abstract
OBJECTIVE:To synthesize 4-hydroxyphenylacetic acid(PHPAA)molecular imprinted polymers(MIPs),and to provide reference for separation and enrichment of PHPAA in urine of cancer patients. METHODS:With PHPAA as template molecule and azobisisobutyronitrile as evocating agent,MIPs was synthesized by precipitation polymerization using 4-vinyl pyridine(4-V),acrylamide(AM),1-vinyl imidazole(1-V)and o-diaminobenzene as functional monomers,ethylene glycol dimethacrylate(EGDMA)and trimethylolpropane trimethacrylate(TRIM)as crosslinking agent. Using scanning electron microscope,infrared spectrum,static adsorption test and molecular recognition performance test used adopted to characterize the structure and performance of MIPs. RESULTS:MIPs1(4-V,EGDMA)microspheres adhered seriously,and MIPs2(AM, EGDMA)microspheres are aggregated. MIPs3(1-V,TRIM),MIPs4(o-diaminobenzene,TRIM)microspheresare were dispersed and had good sphericity. Characteristic absorption peak of PHPAA was not found in infrared spectrum of MIPs. The adsorption capacity of each MIPs was higher than that of blank imprinted polymer without the template molecule,and increased as the increase of template molecular concentration. The adsorption capacity of MIPs3 was significantly higher than that of other MIPs,and its static distribution coefficient(0.14)of PHPAA was higher than that of other structural analogues [PHPA(0.06),TA(0.01)],selective separation factors of PHPAA to PHPA,PHPAA to TA were 2.3,11.5,respectively. CONCLUSIONS:MIPs synthesized with 1-V as functional monomer and TRIM as crosslinking agent has the ability of specific adsorption and selective recognition. It can used as solid phase extractant to separate and enrich low content of PHPAA in the urine of tumor patients.关键词
对羟基苯乙酸/分子印迹聚合物/功能单体/沉淀聚合/静态吸附/分子识别性能Key words
4-Hydroxyphenylacetic acid/Molecular imprinted polymer/Functional monomer/Precipitation polymerization/Static adsorption/Molecular recognition performance分类
化学化工引用本文复制引用
刘伟婷,张媛媛,乾康,高淑婷,万喜,尹小英..肿瘤标志物PHPAA分子印迹聚合物的合成与性能研究[J].中国药房,2018,29(8):1027-1031,5.基金项目
国家自然科学基金资助项目(No.81660652) （No.81660652）
