中国药房2018，Vol.29Issue(8)：1031-1035,5.DOI:10.6039/j.issn.1001-0408.2018.08.05
DiR-PEG-PLGA荧光纳米囊的制备、表征及体外生物相容性评价
Preparation,Characterization and Biocompatibility Evaluation in vitro of DiR-PEG-PLGA Fluorescent Nanocapsules
摘要
Abstract
OBJECTIVE:To prepare and characterize Fluorescent dye 1,1′-octacosyl-3,3,3′,3′-tetramethylindocarbocyanine iodide(DiR)-loading polyethylene glycol-poly lactic-co-glycolic acid(DiR-PEG-PLGA)nanocapsules,and to evaluate its biocompatibility in vitro. METHODS:Using PLGA and PEG-PLGA as carrier,DiR-PEG-PLGA nanocapsules were prepared by modified ultrasonic emulsification method. The particle size,Zeta potential,morphology,stability and fluorescence in vitro of nanocapsules were detected respectively. MTT assay was used to evaluate cytotoxicity in vitro of nanocapsules to human-derived HL7702 hepatocytes,and hemolysis test was carried out to investigate its hemolysis effects. RESULTS:Prepared DiR-PEG-PLGA nanocapsules were spherical with a clear core-shell structure. The average particle size was(507.53 ± 7.87)nm,polydispersity coetficient of particle size was 0.306 1±0.001 5 and Zeta potential was(-35.20±0.92)mV with good stability within 6 months under 4℃. Fluorescence signal intensity(y)of nanocapsules was increased linearly with DiR mass concentration(x)in vitro. The linear eguation was y=0.345 2x+0.433 4(R2=0.997 3).The toxicity of nanocapsules to HL7702 cells was between 0-1 degree,and no hemolytic effect was observed. CONCLUSIONS:The study successfully prepare fluorescent DiR-PEG-PLGA nanocapsules with high biocompatibility in vitro,which is further expected to become a safe optical drug carrier.关键词
纳米囊/超声乳化法/荧光/表征/生物相容性/DiR/聚乙二醇/聚乳酸-羟基乙酸共聚物Key words
Nanocapsules/Ultrasonic emulsification/Fluorescence/Characterization/Biocompatibility/DiR/PEG/PLGA分类
医药卫生引用本文复制引用
程欣,马云淑,柯瑾,陈烁,谢薇,邵长丽,侯安国,张昆,邓林,陈艳五..DiR-PEG-PLGA荧光纳米囊的制备、表征及体外生物相容性评价[J].中国药房,2018,29(8):1031-1035,5.基金项目
国家自然科学基金资助项目(No.81673682) （No.81673682）
云南省科技计划项目[No.2015FB205(-003)、2017FF117(-017)] （-003）
云南省重点实验室培育计划项目(No.2017DG006) （No.2017DG006）
云南省教育厅大学生创新创业训练计划项目(No.2016013) （No.2016013）
