中国药房2018，Vol.29Issue(8)：1044-1048,5.DOI:10.6039/j.issn.1001-0408.2018.08.08
声光可调-近红外光谱法快速测定丹参药材中隐丹参酮的含量
Rapid Determination of Cryptotanshinone in Salvia miltiorrhiza by AOTF-NIDRS
摘要
Abstract
OBJECTIVE:To establish rapid method for content determination of cryptotanshinone in Salvia miltiorrhiza. METHODS:The content of cryptotanshinone in sample was determined by HPLC(as reference value). AOTF-NIDRS combined with PLS was used to establish quantitative correction model for the content of cryptotanshinone in S. miltiorrhiza. According to the results of content determination of cryptotanshinone in samples,35 samples of medicinal material were collected. First-order derivative combined with smoothing filter coefficient method was used to pretreat spectrum,and optimal band range for content determination of cryptotanshinone in sample ranged 1 250-2 150 nm. RESULTS:Methodology validation of content determination of cryptotanshinone in sample was in line with the requirements. Correction mean square deviation of quantitative correction model of cryptotanshinone was 0.014 6,and predicted mean square deviation was 0.022 3,coefficient of association was 0.976 6. The internal verification deviation was 2.41% and the external verification deviation was 4.06%. CONCLUSIONS:This method is rapid,accurate,simple and pollution-free.It can be used for rapid content determination of cryptotanshinone in S.miltiorrhiza.关键词
声光可调-近红外光谱法/偏最小二乘法/丹参/隐丹参酮Key words
AOTF-NIDRS/Partial least square method/Salvia miltiorrhiza/Cryptotanshinone分类
化学化工引用本文复制引用
苏婷,姜文月,邢成,任绪华,李心冬,崔宪利,高陆..声光可调-近红外光谱法快速测定丹参药材中隐丹参酮的含量[J].中国药房,2018,29(8):1044-1048,5.基金项目
吉林省科技发展计划项目(No.20150311003YY、20150301009YY) （No.20150311003YY、20150301009YY）
