中国肿瘤生物治疗杂志2018，Vol.25Issue(3)：221-228,8.DOI:10.3872/j.issn.1007-385x.2018.03.002
Sphk1基因对间充质干细胞诱导结肠癌RKO细胞增殖和迁移的影响
Effects of SphK1 gene on the proliferation and migration of colon cancer RKO cells induced by mesenchymal stem cells
摘要
Abstract
Objective:To investigate the effect of sphingosine kinase 1 (SphK1) knockdown on the proliferation and migration of colon cancer RKO cells induced by mesenchymal stem cells (MSCs).Methods:RKO cells were treated with MSCs conditioned medium (MSC-CM) or control medium (Control-CM),respectively.Cell proliferation was detected by CCK-8 assay.Cell migration ability was tested by Transwell chamber assay.The proteins expression of Ki-67,MMP-2/9,CD44 and CD133 was detected by Western blotting.Then,the expression of SphK1 in RKO cells was suppressed by targeted gene lentivirus shRNA vector transfection.The effects of SphK1 knockdown on the proliferation,migration and protein expressions of Ki-67,MMP-2/9,CD44 and CD 133 of RKO cells induced by MSC-CM were observed.Results:The RKO cells proliferation was promoted by MSC-CM in a time-dependent manner;moreover (P＜0.05),the migration ability of cells was significantly enhanced after being treated with MSC-CM(P＜0.01).In addition,MSC-CM significantly increased the protein expressions of Ki-67,MMP-2/9,CD44 and CD133(all P＜0.05 or P＜0.01).Lentiviral ShRNA vector transfection could significantly inhibit the expression of SphK1.Down-regulation of SphK1 significantly inhibited the proliferation,migration and protein expressions of Ki-67,MMP-2/9,CD44 and CD133 of RKO cells induced by MSC-CM(all P＜0.05 or P＜0.01).Conclusion:MSC-CM promotes the proliferation and migration of colon cancer RKO cells.Down-regulation of SphK1 reverses the cell proliferation and migration induced by MSC-CM via inhibiting the expression of MMP-2/9,CD44 and CD 133.关键词
鞘氨醇激酶1基因/间充质干细胞/结肠癌/RKO细胞/增殖/迁移Key words
sphingosine kinase 1 gene (SphK1)/mesenchymal stem cell(MSC)/colon cancer/RKO cell/proliferation/migration分类
医药卫生引用本文复制引用
伍文红,刘诗权,符振华,覃蒙斌,徐春燕,朱丽叶,黄杰安..Sphk1基因对间充质干细胞诱导结肠癌RKO细胞增殖和迁移的影响[J].中国肿瘤生物治疗杂志,2018,25(3):221-228,8.基金项目
国家自然科学基金资助项目(No.81460380) （No.81460380）
广西自然科学基金项目(No.2017GXNSFAA198019) （No.2017GXNSFAA198019）
广西研究生教育创新计划项目(No.YCBZ2017035).Project supported by the National Natural Science Foundation of China (No.81460380),the Natural Science Foundation of Guangxi (No.2017GXNSFAA198019),and the Guangxi Provincial Foundation for Innovation Project of Graduate Education(No.YCBZ2017035) （No.YCBZ2017035）
