中国肿瘤生物治疗杂志2018，Vol.25Issue(3)：236-239,4.DOI:10.3872/j.issn.1007-385x.2018.03.004
四环素调控livin RNA干扰系统对肺癌移植瘤生长的影响
Suppressive effect of Tet-on mediated livin RNA interference on growth of lung carcinoma xenegraft in a rat model
摘要
Abstract
Objective:To investigate he effect of tetracycline-(Tet-on) mediated livin RNA interference on growth of lung carcinoma xenegrafts,and find a better regulatory way to interfere the development on lung cancer.Methods:livin shRNA lentiviral vectors were constructed;and the lung cancer A549 cells were subcutaneously injected into right upper back of nude mice to establish xenegraft model.The livin shRNA lentiviral vectors were injected into xenografts to interfere the expression of livin,then tetracycline was injected intraperitoneally for the induction.The suppressive effect of Tet-on mediated livin RNA interference efficiency was investigated and lung cancer xenograft development was observed.Results:After the induction with Tet-on,livin gene expression was significantly inhibited by livin shRNA compared with the control group and Tet-on-NC group;the xenograft volume in Tet-on-livin shRNA group was significantly smaller than that in control group and Tet-on-NC group ([5.31 ±0.86]g vs [8.22±0.63]g and [7.17±0.54] g,P＜0.05).Moreover,little body toxicity was observed and no nude mice died in this study.Conclusion:The Tet-on mediated livin shRNA could suppress the growth of lung cancer development with good targeting and controllable characteristics,which might provide a potent tool for treating lung cancer with livin protein as target.关键词
livin基因/四环素调控/RNA干扰/肺癌/A549细胞/动物模型Key words
livin gene/Tet-on mediated regulation/livin RNA interfere/lung carcinoma/A549 cell line/animal model分类
医药卫生引用本文复制引用
李鸿茹,涂洵崴,翁丽红,陈愉生..四环素调控livin RNA干扰系统对肺癌移植瘤生长的影响[J].中国肿瘤生物治疗杂志,2018,25(3):236-239,4.基金项目
福建省自然科学基金资助项目(No.2015J01376) （No.2015J01376）
福建省立医院优秀青年医师项目(No.2014YNQN03).Project supported by the Natural Science Foundation of Fujian Province (No.2015J01376),and the Foundation of Foujian Provincial Hospital for Excellent Young Physicians (No.2014YNQN03) （No.2014YNQN03）
