中国肿瘤生物治疗杂志2018，Vol.25Issue(3)：252-257,6.DOI:10.3872/j.issn.1007-385x.2018.03.007
乌苏酸联合吉西他滨对胰腺癌PANC-1细胞增殖和凋亡的影响
Effect of ursolic acid combined with gemcitabine on proliferation and apoptosis of pancreatic cancer PANC-1 cells
摘要
Abstract
Objective:To study the effects ofursolic acid cooperated with gemcitabine on proliferation and apoptosis of pancreatic cancer PANC-1 cells.Methods:Human pancreatic cancer cell line PANC-1 was cultured in vitro with ursolic acid and gemcitabine respectively;and MTT assay was used to determine the IC50 of ursolic acid and gemcitabine,thus obtaining the best drug concentration.Ursolic acid (2 μmol/L) and gemcitabine (0.2 μmol/L) alone or in combination was used to treat PANC-1 cells;trypan blue assay was used to test cell viability,and PI staining was used to examine the cell apoptosis;wound healing was used to detect the proliferation and migration of PANC-1 cells.The protein expressions of P-JNK,Bcl-2,IL-6,P-Stat 3,NF-κB and Cox-2 in cells of each treatment group were detected using Western blotting.Results:Both ursolic acid and gemcitabine could significantly inhibit the proliferation of PANC-1 cells,and the IC50 is 13.67 and 2.78 μmol/L,respectively;and the final concentrations were determined at 2 and 0.2 μmol/L for ursolic acid and gemcitabine,respectively.Compared with single drug treatment,the combined treatment exerted a more prominent cell proliferation inhibition effect ([46.47±5.07]％ vs [78.38±8.65]％,[76.12±3.23]％,all P＜0.05),apoptosis-induction effect ([39.78±7.01]％ vs [20.35±8.51]％,[20.35±8.51]％,all P＜0.01) and migration inhibition effect (P＜0.01) on PANC-1 cells.Western blotting showed that the combined treatment strongly inhibited Bcl-2 and IL-6 expression,accelerated P-JNK protein expression compared with single drug treatment.Conclusion:The synergistic effect of ursolic acid and gemcitabine enhanced the inhibition on proliferation,migration,and promoted cell apoptosis of human pancreatic cancer cell line PANC-1,the mechanism may be associated with inhibition of Bcl-2,I1-6,P-stat 3 proteins and promotion of P-JNK protein.关键词
胰腺癌/乌苏酸/吉西他滨/增殖/迁移/凋亡Key words
pancreatic cancer/ursolic acid/gemcitabine/proliferation/migration/apoptosis分类
医药卫生引用本文复制引用
江森,郑希,何燕,Tina LIAO,张焜..乌苏酸联合吉西他滨对胰腺癌PANC-1细胞增殖和凋亡的影响[J].中国肿瘤生物治疗杂志,2018,25(3):252-257,6.基金项目
国家自然科学基金资助项目(No.81272452).Project supported by the National Nature Science Foundation of China (No.81272452) （No.81272452）
