中国肿瘤生物治疗杂志2018，Vol.25Issue(3)：263-269,7.DOI:10.3872/j.issn.1007-385x.2018.03.009
FBXW7基因点突变对结直肠癌HCT-116细胞的增殖、迁移、侵袭和抗凋亡能力的影响
The effect of FBXW7 gene point mutation to colorectal cancer HCT-116 cells' proliferation, migration, invasion, and its apoptosis resistance
摘要
Abstract
Objective:To investigate the impact ofFBXW7 gene mutation on cell proliferation,apoptosis,migration,and invasion processes of colorectal cancer HCT-116 cell line.Methods:Recombinant plasmids carrying wild-type and mutant-type FBXW7 SNP were constructed and transfected into HCT-116 cell line;the FBXW7 protein expression level in HCT-116 strains after transfection was detected by Western blotting.Subsequently,cell proliferation capacity was tested by CCK-8 assay;tumor cell colony formation ability was tested by HTCA;cell apoptosis function was tested by FCM;cell migration and invasion were tested by scratch assay and Transwell assay,respectively.Results:Higher HBXW7 protein expression level was detected in HCT-116 strain transfected with wild-type HBXW7 in comparison to the control group (strains transfected with mutant-type HBXW7),negative-control (strains transfected with empty plasmids),and blank-control (strains untransfected) (all P＜0.05).Compared with the other groups,strains transfected with wildtype HBXW7 exhibited significantly reduced proliferation,colony formation,migration and invasion ability (all P＜0.05),but obviously increased apoptosis rate (P＜0.05).Conclusion:FBXW7 gene mutation can down-regulate its protein expression,and further promote the proliferation,migration and invasion as well as inhibit the apoptosis ofHCT-116 cells.关键词
FBXW7基因/基因突变/结直肠癌/HCT-116细胞/泛素蛋白酶体系统Key words
FBXW7 gene/gene mutation/colorectal cancer/HCT-116 cell/ubiquitin-proteasome system分类
医药卫生引用本文复制引用
张百川,程勇,王祥峰,唐康,王五艺..FBXW7基因点突变对结直肠癌HCT-116细胞的增殖、迁移、侵袭和抗凋亡能力的影响[J].中国肿瘤生物治疗杂志,2018,25(3):263-269,7.基金项目
重庆市科委自然科学基金项目(No.cstc2012jjA10038). Project supported by the Natural Science Foundation of Chongqing Municipal-Science and Technology Commission (No.cstc2012jjA10038) （No.cstc2012jjA10038）
