中国中西医结合外科杂志2018，Vol.24Issue(2)：134-140,7.DOI:10.3969/j.issn.1007-6948.2018.02.002
急性胰腺炎血液和胆汁培养的病原菌分布及耐药性分析
Analysis on Pathogen Species Distribution and Drug Resistance in Blood and Bile Culture of Acute Pancreatitis Patients
摘要
Abstract
Objective To analyze the species distribution and resistant characteristics of pathogenic bacteria isolated from blood and bile cultures of patients with acute pancreatitis. Methods Blood and bile samples of patients with acute pancreatitis were cultivated and continuously monitored by BacT/Alert 3 D Automated Blood Culture System and the bacteria in the positive bottles were purified by being transferred timely to plate culture. The identifications of bacteria and drug tests were performed by Vitek 2 Compact Bacterial Automatic Analyzer. Results There were 245 strains of bacteria cultured in 1195 blood and 408 bile samples of patients with acute pancreatitis. Blood and bile culture detection rates were 9.2% and 20.8%, respectively. The six species detected in blood culture were coagulase negative Staphylococcus (CNS), Escherichia coli, Klebsiella pneumoniae, nearly smooth Candida, Acinetobacter baumannii, and Enterococcus faecium. The six species detected in bile culture were E. coli, E. faecium, K. pneumoniae, Enterococcus faecalis, CNS and A. baumannii. In blood and bile cultures, the detection rates of methicillin-resistant CNS were 91.1% and 60.0%, respectively. The detection rates of E. coli producing ESBLs were 52.0% and 66.7%, respectively. The detection rates of K. pneumoniae producing ESBLs were 45.5% and 70.0%, respectively. The resistant rates of A. baumannii to imipenem were 80.0% and 50.0%, respectively. Conclusion The pathogen detection rate is greater in bile culture than that in blood culture. CNS and E. coli were predominant pathogenic bacteria of blood and bile samples of patients with acute pancreatitis, respectively. The bacteria in blood and bile samples have different drug resistance.关键词
急性胰腺炎/血液培养/胆汁培养/病原菌分布/耐药性Key words
Acute pancreatitis/blood culture/bile culture/pathogenic bacteria distribution/drug resistance分类
医药卫生引用本文复制引用
陈莎燕,高雪,白景瑞,孙兰菊,陈明慧,房杰,常艳敏..急性胰腺炎血液和胆汁培养的病原菌分布及耐药性分析[J].中国中西医结合外科杂志,2018,24(2):134-140,7.基金项目
国家自然科学基金(81703846) （81703846）
