中国中西医结合外科杂志2018，Vol.24Issue(2)：141-145,5.DOI:10.3969/j.issn.1007-6948.2018.02.003
miR-449c在胃癌中的表达
Expression of miR-449c in Gastric Cancer
摘要
Abstract
Objective To detect the miR-449c expression in gastric cancer(GC) and discuss the relationship between miR-449c expression and carcinogenesis and prognosis of GC. Methods A total of 32 patients with GC were selected as researching subjects. Real-time fluorescence quantitative PCR(Real-time PCR) was used to detect the expression of miR-449c in normal gastric tissue(NGT), gastric cancer tissues(GC) and GC cell lines. miR-449c mimics and inhibitors were trasfected into GC cells according to the instructions of Lipofectamine 2000. Cell viability was detected by MTT and cell apoptosis was detected by FACS. The survival rate was analyzed between the patients with high expression of miR-449 c and the patients with low expression of miR-449 c. Results The results showed that the expression level of miR-449c of GC (0.36±0.28) was significantly lower than that of NGT group (1.01±0.14, P<0.05). The expression level of miR-449c in AGS, SNU-1, SNU-5 and SNU-16 cell lines (0.39±0.08, 0.33±0.07, 0.25±0.06, 0.22±0.04, respectively) was significantly lower than that in NGT(P<0.05). The expression level of miR-449c (8.49±2.50) in SNU-16 cell line transfected with miR-449c mimics was significantly higher than that of SNU-16 cells transfected with scrambled sequences(scr) (P<0.05). Cell viability at second day (0.80 ± 0.10)and third day (0.90 ± 0.12) was significantly lower than that of SNU-16 cells transfected with scr(P<0.05). The apoptosis rate (80.12±9.01) was significantly higher than that of SNU-16 cells transfected with scr(P<0.05). The expression level of miR-449c in AGC cells transfected with miR-449c inhibitor (3.00±0.50) was significantly lower than that of AGC cells transfected with scr(P<0.05).Cell viability at second day (1.20±0.14) and third day (1.70±0.18) was significantly higher than that of AGC cells transfected with scr(P<0.05). The apoptosis rate (8.00±1.00) was significantly lower than that of AGC cells transfected with scr(P<0.05). Followed-up for 30 months, survival rate (50 %) in the patients with high miR-449c expression level was significantly higher than that of low miR-449c expression level (P<0.05). Conclusion miR-449c expressed lower in GC tissue. It could inhibit cell growth and promote apoptosis. miR-449c expression was closely correlation with the survival rate of the GC patients.关键词
miR-449c/胃癌/表达Key words
miR-449c/gastric cancer/expression分类
医药卫生引用本文复制引用
吴志明,黄洪军,孟兴成,储修峰,王焕根,邱海江..miR-449c在胃癌中的表达[J].中国中西医结合外科杂志,2018,24(2):141-145,5.基金项目
浙江省医学会临床科研基金(2016ZYC-A90) （2016ZYC-A90）
