中成药2018，Vol.40Issue(3)：505-511,7.DOI:10.3969/j.issn.1001-1528.2018.03.001
藏药灰兜巴对STZ诱导的Ⅱ型糖尿病肾病小鼠的肾脏保护作用
Protection of Huidouba, a Tibetan medicine on renal injury in STZ-induced type Ⅱ diabetic mice
摘要
Abstract
AIM To study the efficacy of different extractions of Huidouba,a Tibetan medicine to mice with type Ⅱ diabetic kidney disease (DKD).METHODS The type Ⅱ DKD mice models induced by the injection of streptozotocin (STZ) on base of high glucose and high fat diet subsequently treated with different extractions of Huidouba were studied to investigate the protective effects of Huidouba on renal injury.The mice were given high dose (200 mg/kg) and low dose (100 mg/kg) of Huidouba ethanol extract,Huidouba water extract and Huidouba polysaccharide respectively for 8 weeks.Their serum creatinine (SCr),blood urea nitrogen (BUN),urine protein (mg/24 h),glycosylated serum protein (Gsp) and advanced glycation end products (AGEs),their histological changes of renal pathology were detected and analyzed.Meanwhile their protein levels of fibronectin (FN),intercellular adhesion factor (ICAM-1) and transforming growth factor (TGF-β1) were identified by Western blot.RESULTS The different extractions of Huidouba,demonstrating a portfolio of capacity in decreasing levels of serum glucose and AGEs,inhibiting the expression of target protein,alleviating the glomerular basement membrane thickening,improving mesangial matrix,renal tubular and epithelial cells degeneration and other pathological conditions,improved the renal function of DKD mice.CONCLUSION The three extractions of Huidouba highlight the renal improvements in type Ⅱ DKD mice.关键词
藏药/灰兜巴/糖尿病肾病(DKD)/终末糖基化产物(AGEs)/转化生长因子(TGF-β1)Key words
Tibetan medicine/Huidouba/diabetic kidney disease (DKD)/advanced glycation end products (AGEs)/transforming growth factor (TGF-β1)分类
医药卫生引用本文复制引用
周园里,贾晓斌,刘继平,封亮..藏药灰兜巴对STZ诱导的Ⅱ型糖尿病肾病小鼠的肾脏保护作用[J].中成药,2018,40(3):505-511,7.基金项目
江苏省医学创新团队(CXTDB2017003) （CXTDB2017003）
江苏省第十三批“六大人才高峰”创新人才团队项目(SWYY-CXTD-004) （SWYY-CXTD-004）
