中成药2018，Vol.40Issue(3)：537-543,7.DOI:10.3969/j.issn.1001-1528.2018.03.006
泽兰甲醇提取物对慢性软组织损伤模型大鼠的影响及其机制
Effect and mechanism of methanolic extract of Eupatorium to model rats with chronic muscle injury
高渐联 1李汉伟 2邓智建 3卢乙众 1张鑫 1王金彩 1贾奎1
作者信息
- 1. 新乡医学院第一附属医院,河南新乡453100
- 2. 河南省神经修复重点实验室,河南新乡453100
- 3. 河南中医药大学,河南郑州450046
- 折叠
摘要
Abstract
AIM To investigate the effect and mechanism of methanolic extract of Eupatorium (MEOE) to model rats with chronic soft tissue injury.METHODS The model rats were established by mechanical injury and a subsequent two-week normal feeding for respective administration of high,medium and small dosage of MEOE once a day successively for 14 days.An array of indices,the level of superoxide dismutase (SOD),malondialdehyde (MDA),prostaglandin E2 (PGE2),nitric oxide (NO),interleukin-6 (IL-6) and histamine,the expression of tumor necrosis factor-alpha (TNF-α),nitric oxide (NO) and Collagen-Ⅰ/Ⅲ,and the activity of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) were measured to analyze the effect of MEOE to model rats with chronic muscle injury.RESULTS MEOE resulted in apparent reduction of contents of MDA,PGE2 and NO,and the levels of TNF-α and IL-6 in muscular tissue (P ＜ 0.05),significantly increased of the SOD in muscular tissue (P ＜ 0.01),a remarkably inhibited expression of the tissue Collagen-Ⅰ/Ⅲ protein (P ＜ 0.01),and significantly improved activity of tissue VEGF and bFGF (P ＜ 0.01).CONCLUSION The certain therapeutic effects of MEOE to rats with chronic muscle injury may correlate with its influence to the levels of inflammatory factors inhibition,the oxidative stress relief,the overexpression of collagen-Ⅰ/Ⅲ inhibition,the VEGF and bFGF activity improvement,and the time spare from the repairing.关键词
泽兰/慢性软组织损伤/炎症细胞因子/氧化应激/Collagen-Ⅰ/Ⅲ/VEGF/bFGFKey words
Eupatorium/chronic soft tissue injury/inflammatory cytokines/oxidative stress/Collagen-Ⅰ/Ⅲ/VEGF/bFGF分类
医药卫生引用本文复制引用
高渐联,李汉伟,邓智建,卢乙众,张鑫,王金彩,贾奎..泽兰甲醇提取物对慢性软组织损伤模型大鼠的影响及其机制[J].中成药,2018,40(3):537-543,7.
