中成药2018，Vol.40Issue(3)：549-553,5.DOI:10.3969/j.issn.1001-1528.2018.03.008
姜黄素联合雷公藤红素体内外抗胃癌作用评价
In vitro and in vivo anti-tumor effects of a combination therapy of curcumin and celastrol to gastric cancer
苗金钰 1佘君 2张玉磊 1孙万森1
作者信息
- 1. 西安交通大学校医院,陕西西安710049
- 2. 西安交通大学第二附属医院中医科,陕西西安710004
- 折叠
摘要
Abstract
AIM To evaluate anti-gastric cancer effects and benefits of the combination therapy of celastrol and curcumin in vitro and in vivo.METHODS Curcumin,celastrol and curcumin + celastrol applied to BGC823 gastric cancer cells had their impact on the cells growth and apoptosis in vitro checked by CCK-8 assay and flow cytometry of AnnexinV-FITC/PI;and their in vivo anti-tumor effect,systemic toxicity,and influence to the survival time evaluated by using BGC823-bearing gastric tumor mouse models induced by subcutaneous implantation.RESULTS IC50 of curcumin,celastrol and curcumin + celastrol on BGC823 cell were found to be at (57.47 ± 5.22),(2.11 ±0.07) and (0.22 ±0.01) μmol/L,respectively.Curcumin + celastrol achieved a most optimal BGC823 cell apoptosis rate of (73.07 ±3.82)％ at concentrations of (10 mol/L +2 mol/L);and they left a positive impact on the BGC823 gastric tumor-bearing mice,given a (64.87 ±4.16)％ inhibitory rate,and a 50.5-day median survival time.Additionally,the combination therapy demonstrated its less side effects in terms of the body weight,spleen and liver of the nude mice,and significantly improved serum concentrations of TNF-α and IL-6.CONCLUSION Combination of curcumin and celastrol shows significantly synergistic anti-gastric cancer effects in vitro and in vivo.关键词
姜黄素/雷公藤红素/抗胃癌/联合治疗Key words
curcumin/celastrol/anti-gastric cancer/combination therapy分类
医药卫生引用本文复制引用
苗金钰,佘君,张玉磊,孙万森..姜黄素联合雷公藤红素体内外抗胃癌作用评价[J].中成药,2018,40(3):549-553,5.
