中成药2018，Vol.40Issue(3)：613-617,5.DOI:10.3969/j.issn.1001-1528.2018.03.021
康复新液HPLC指纹图谱建立及6种成分测定
Establishment of HPLC fingerprints of Kangfuxin Liquid and determintion of six constituents
摘要
Abstract
AIM To establish the HPLC fingerprints of Kangfuxin Liquid (extract of Periplaneta americana L.) and to determine the contents of six constituents.METHODS The analysis of this drug was performed on a TOSOH TSK-GEL ODS column (250 mm × 4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-water (containing 0.07％ acetic acid) flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 280 nm.RESULTS There were twenty-four common peaks in the fingerprints of ten batches of samples (Ⅰ-Ⅹ) with the similarities of 0.932-0.993 (except for sample Ⅰ).Uracil,hypoxanthine,xanthine,inosine,protocatechuic acid and Cyclo (Gly-Tyr) showed good linear relationships within the ranges of 3.460-173.0,3.960-198.0,3.596-179.8,1.338-66.9,3.672-183.6 and 3.552-177.6 μg/mL,whose average recoveries (RSDS) were99.8％ (2.65％),98.0％ (2.55％),99.7％ (1.59％),100.7％ (2.80％),102.0％ (2.09％) and 99.6％ (1.88％),respectively.CONCLUSION This accurate,stable and simple method can be used for the quality control of Kangfuxin Liquid.关键词
康复新液/HPLC指纹图谱/尿嘧啶/次黄嘌呤/黄嘌呤/肌苷/原儿茶酸/环(甘氨酰-酪氨酸)Key words
Kangfuxin Liquid/HPLC fingerprints/uracil/hypoxanthine/xanthine/inosine/protocatechuic acid/Cyclo (Gly-Tyr)分类
医药卫生引用本文复制引用
吕娜,王钧篪,陈丽华,沈连刚,李广志,斯建勇..康复新液HPLC指纹图谱建立及6种成分测定[J].中成药,2018,40(3):613-617,5.基金项目
“十二五”国家科技重大创新药物专项(2011ZX09102-101-01,2012ZX09301-002-001) （2011ZX09102-101-01,2012ZX09301-002-001）
