中成药2018，Vol.40Issue(3)：632-637,6.DOI:10.3969/j.issn.1001-1528.2018.03.026
扭肚藤UPLC指纹图谱建立及2种成分测定
Establishment of UPLC fingerprints of Jasminum elongatum and determination of two constituents
摘要
Abstract
AIM To establish the UPLC fingerprints of Jasminum elongatum (Bergium) Wild.and to determine the contents of isochlorogenic acid B and ethylcaffeate.METHODS The analysis of methanol extract of J.elongatum was developed on a 25 ℃ thermostatic Agilent Ecplise XDB-C18 column (2.1 mm × 100 mm,1.7 μm),with the mobile phase comprising of acetonitrile-0.1％ methanoic acid flowing at 0.5 mL/min in a gradient elution manner,and the detection wavelength was set at 260 nm.RESULTS There were eighteen common peaks in the fingerprints of ten batches of samples,with the similarities of more than 0.85.Isochlorogenic acid B and ethylcaffeate showed good linear relationships within the ranges of 7.67-38.35 μg/mL (R2 =0.999 4),9.60-96.0 μg/mL (R2 =0.999 7),whose average recoveries were 98.61％,99.09％ with the RSDs of 0.84％,1.25％,respectively.CONCLUSION This stable and reliable method can be used for the quality control of J.elongatum.关键词
扭肚藤/UPLC指纹图谱/异绿原酸B/咖啡酸甲酯Key words
Jasminum elongatum (Bergium) Wild./UPLC fingerprints/isochlorogenic acid B/methylcaffeate分类
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胥爱丽,毕晓黎,刘布鸣,江洁怡,陈昭,丁平..扭肚藤UPLC指纹图谱建立及2种成分测定[J].中成药,2018,40(3):632-637,6.基金项目
广西中药质量标准研究重点实验室开放课题资助项目(201402) （201402）
广东省科技计划项目(2017A070701017,2015B070701010) （2017A070701017,2015B070701010）
佛山市科技创新专项资金(2013GQ100032) （2013GQ100032）
