中成药2018，Vol.40Issue(3)：653-658,6.DOI:10.3969/j.issn.1001-1528.2018.03.030
多元统计分析比较宛艾及31个产地艾叶HPLC指纹图谱
Analysis and comparison of HPLC fingerprints of wanai and Artemisiae argyi from thirty-one growing areas by multistatistical
摘要
Abstract
AIM To analyze and compare HPLC fingerprints of wanai and Artemisiae argyi Levi.et Vant from thirty-one growing areas by multistatistical.METHODS The analysis of 80％ methanol extract of A.argyi was developed on a 30 ℃ thermostatic Agilent TC-C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrile-water (containing 0.2％ methanoic acid) flowing at 1 mL/min in a gradient elution manner,and the detection wavelength was set at 330 nm.RESULTS There were eighteen,twenty-five common peaks in the fingerprints of thirty-one batches of A.argyi,fifteen batches of wanai,respectively,with the similarities all more than 0.900.The similarities of thirty-one batches of samples from different growing area were good and together as a category except samples from Dengzhou city,Luohe city and Anhui province.Fifteen batches of wanai samples got together with Qiai among them.The cumulative contribution rate of the four principal components from A.argyi was 86.049％.Twelve batches of wanai samples had higher scores than Qiai.CONCLUSION This stable and reliable method can be used for the quality control of A.argyi.关键词
艾叶/宛艾/多元统计/HPLC/相似度分析/聚类分析/主成分分析Key words
Artemisiae argyi Levl.et Vant/wanai/multistatistical/HPLC fingerprints/similarity analysis/cluster analysis/principal component analysis分类
医药卫生引用本文复制引用
王宇卿,庄果,张须学,耿榕徽..多元统计分析比较宛艾及31个产地艾叶HPLC指纹图谱[J].中成药,2018,40(3):653-658,6.基金项目
河南省博士后科研项目(2014110) （2014110）
河南省基础与前沿技术研究(152300410181) （152300410181）
河南省中医药科学研究专项课题(2015ZY02005) （2015ZY02005）
