安徽农业科学2018，Vol.46Issue(6)：1-4,32,5.
基于NHS基团修饰的副溶血性弧菌免疫磁珠制备及其富集性能评价
Preparation of Vibrio parahaemolyticus Immunomagnetic Beads Based on NHS Group and Evaluation of Enrichment Capacity
摘要
Abstract
[Objective] The aim was to prepare Vibrio parahaemolyticus immunomagnetic beads (IMBvp) based on NHS group and evaluate the enrichment capacity.[Method] The best coupling conditions were decided by conjugate ratio,like conditions range from magnetic beads particle diameter,coupling time and the adding amount of antibody.The best enrichment conditions were decided by capture rates,like conditions range from pH,incubation time and the adding amount of IMBvp.The enrichment capacity of IMBvp was evaluated according to specificity,sensitivity,precision and stability.[Result] The IMBvp was prepared by chemical bond,which combined 0.1 mL of NHS bead with size N2 and 0.2 mL of Vibrio parahaemolyticus antiserum.IMBvp was placed into the bacterial suspension which pH was 7.0-7.4.Volume addition of was IMBvp 0.4 mL.They were incubation 2 h together.The method was involved in some advantages,high specificity,sensitivity and accuracy.The detection limit was 15 cfu/50 mL.The CVs of intra and inter assay were between 3％ and 6％.The accuracy was greater than 91％.[Conclusion] The above results demonstrate that homemade IMBvp has good enrichment performance.Experiment results show that it is an effective method,convenience,low cost and quickness.关键词
NHS基团/副溶血弧菌/免疫磁珠/富集Key words
NHS/Group Vibrio parahaemolyticus/Immunomagnetic beads/Enrichment分类
农业科技引用本文复制引用
刘阳,刘文侠,孙力军,施琦,邱妹,邓旗..基于NHS基团修饰的副溶血性弧菌免疫磁珠制备及其富集性能评价[J].安徽农业科学,2018,46(6):1-4,32,5.基金项目
国家自然科学基金项目(31371746) （31371746）
广东省质量技术监督局科技项目(2015ZZ02). （2015ZZ02）
