安徽农业科学2018，Vol.46Issue(6)：87-89,185,4.
湖羊GDF9基因多态性和产羔性能的关系
The Relationship between GDF9 Gene Polymorphism and Lambing Performance in the Hu Sheep
摘要
Abstract
[Objective] To study the relationship between GDF9 gene polymorphism and lambing performance in the Hu sheep.[Method] Hu sheep's GDF9 gene polymorphism was detected by PCR-SSCP.The effect of polymorphism on the lambing performance of Hu sheep was analyzed.[Result] Three genotypes were detected and defined as GG,AG,AA.Genotype sequencing showed that the base A was replaced by base G at 152bp.GG genotype frequency,AG genotype frequency and AA genotype frequency were calculated,and the results were 0.107,0.883 and 0.010.The average number of two litters of GG,AG,AA genotypes were 2.068,2.025 and 1.500,respectively.The three genotypes had no effect on the first litter size.There was significant difference between GG,AG and AA in second litter and the GDF9 gene had an effect on the second litter size.From the average number of two litters,the results of variance analysis were not significant,but it also could be seen from the date that the number of litter size of GG and AG genotypes was relatively higher than litter size of AA genotypes.[Conclusion] GDF9 gene is necessary for normal breeding of sheep,and it has a certain effect on the litter size of Hu sheep.关键词
湖羊/GDF9基因/PCR-SSCP/产羔数Key words
Hu sheep/GDF9 gene/PCR-SSCP/Litter size分类
农业科技引用本文复制引用
顾祝荣,郭海燕,王强,李拥军,瞿静雯,杨媛..湖羊GDF9基因多态性和产羔性能的关系[J].安徽农业科学,2018,46(6):87-89,185,4.基金项目
江苏省苏北科技专项“湖羊健康高效养殖配套技术研究与应用”(BN2016076). （BN2016076）
