安徽农业科学2018，Vol.46Issue(6)：157-161,5.
UPLC-MS/MS法测定元胡中4种有机磷农药残留的不确定度评定
Uncertainty Evaluation of Determination of Organic Organophosphorus Residues in Corydalis yanhusuo by UPLC-MS/MS
摘要
Abstract
[Objective] This study aimed to evaluate the uncertainty of measurement in the determination of organic organophosphorus residues in Corydalis yanhusuo by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).[Method] According to JJF 1135-2005.Evaluation of Uncertainty in Chemical Analysis Measurement and JJF 1059.1-2012 Evaluation and Expression of Uncertainty in Measurement,a mathematical model was established for uncertainty evaluation.The sources of uncertainty that may be introduced were analyzed and each component of uncertainty was quantified for the calculation of the combined uncertainty.[Result] The expanded uncertainty of (0.048 57 ±0.011 55) μg/kg (k =2) was obtained for the determination of the presence of 0.048 57 μg/kg malathion;the uncertainty of (0.043 14 ±0.011 02) μg kg (k =2) was obtained for the determination of the presence of 0.043 14 μg/kg isocarbophos;the uncertainty of (0.065 34 ±0.016 41) μg/kg (k =2) was obtained for the determination of the presence of 0.065 34 μg/kg omethoate;the uncertainty of (0.058 36 ±0.013 02)μg/kg (k =2) was obtained for the determination of the presence of 0.058 36 μg/kg triazophos.[Conclusion] The preparation of standard solution,curve fitting and measurement repeatability were found to be the main sources of uncertainty.关键词
不确定度评定/有机磷农药残留/元胡/超高效液相色谱-串联质谱法(UPLC-MS/MS)Key words
Uncertainty evaluation/Organic organophosphorus residues/Corydalis yanhusuo/Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)分类
农业科技引用本文复制引用
刁银军,刘莉,肖珊美,杨德毅,洪佳琪,沈一蕾..UPLC-MS/MS法测定元胡中4种有机磷农药残留的不确定度评定[J].安徽农业科学,2018,46(6):157-161,5.基金项目
浙江省公益技术应用研究分析测试项目(2016C37009). （2016C37009）
