安徽中医药大学学报2018，Vol.37Issue(1)：58-62,5.DOI:10.3969/j.issn.2095-7246.2018.01.018
益气健脾通便方对慢传输型便秘大鼠Cajal间质细胞及一氧化氮、一氧化氮合酶的影响
Effect of qi-tonifying, spleen-strengthening, and bowel-relaxing prescription on Cajal interstitial cells, nitric oxide, and nitric oxide synthase in rats with slow transit constipation
摘要
Abstract
Objective To investigate the effect of qi-tonifying,spleen-strengthening,and bowel-relaxing prescription on Cajal interstitial cells (ICC),nitric oxide (NO),and nitric oxide synthase (NOS) in rats with slow transit constipation (STC) and possible mechanism of action.Methods A total of 60 specific pathogen-free male Wistar rats aged 8 weeks were randomly divided into normal group,model group,low-,medium-,and high-dose qi-tonifying,spleen-strengthening,and bowel-relaxing prescription groups,and mosapride group,with 10 rats in each group.Compound diphenoxylate was given by gavage to establish a rat model of STC.After the model was established,the drugs were given for 4 consecutive weeks.The general status of rats was observed,and the number of fecal pellets,fecal water content,intestinal propulsion rate,expression of ICC,and content of NO and NOS were measured.Results The qi-tonifying,spleen-strengthening,and bowel-relaxing prescription improved the general status of rats,increased the number of fecal pellets,fecal water content,intestinal propulsion rate,and the expression of ICC in the colon tissue,and reduced the content of neurotransmitters NO and NOS (all P＜0.05).Conclusion The pathogenesis of STC may be associated with the reduction in the expression of ICC and the increase in the levels of NO and NOS.关键词
慢传输型便秘/益气健脾通便方/Cajal间质细胞/一氧化氮/一氧化氮合酶Key words
Slow transit constipation/Qi-tonifying, spleen-strengthening, and bowel-relaxing prescription/Cajal interstitial cell/Nitric oxide/Nitric oxide synthase分类
医药卫生引用本文复制引用
王建民,王培森,唐冉..益气健脾通便方对慢传输型便秘大鼠Cajal间质细胞及一氧化氮、一氧化氮合酶的影响[J].安徽中医药大学学报,2018,37(1):58-62,5.基金项目
安徽省卫生计生委中医药科研课题(2016zy02) （2016zy02）
