安徽中医药大学学报2018，Vol.37Issue(1)：67-71,5.DOI:10.3969/j.issn.2095-7246.2018.01.020
新鱼腥草素钠对小鼠鲍曼不动杆菌肺感染模型的保护作用
Protective Effect of Sodium New Houttuyfonate Against Acinetobacter baumannii Pulmonary Infection in Mice
摘要
Abstract
Objective To establish a mouse model of pneumonia induced by Acinetobacter baumannii,and to investigate the protective effect of sodium new houttuyfonate against Acinetobacter baumannii pulmonary infection.Methods Mice were divided into control group,pneumonia model group,and low-and highdose sodium new houttuyfonate groups.C57BL/6 mice were pretreated with cyclophosphamide on days 4 and 1 before surgery,and since day 3 before treatment,the mice in the low-and high-dose sodium new houttuyfonate groups were given sodium new houttuyfonate.The Acinetobacter baumannii strain isolated in the clinical ICU was used to prepare fresh bacterial solution,and airway inoculation was performed to establish a mouse model of Acinetobacter baumannii pulmonary infection.Bacterial count in the lung and blood was determined at 24 and 72 hours after inoculation.Hematoxylin and eosin staining was used to observe inflammatory changes in lung tissue.The numbers of leukocytes and neutrophils and the levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in bronchoalveolar lavage fluid (BALF) were measured.Results There was a significant increase in the number of bacteria in the lung and blood after the inoculation with Acinetobacter baumannii in mice (P＜0.05),and the mice had serious inflammatory response and tissue injury,as well as significant increases in leukocyte count and levels of TNF-α and IL-1β in BALF (P＜0.05).After the treatment with sodium new houttuyfonate,compared with the pneumonia model group,the low-and high-dose sodium new houttuyfonate groups had significantly lower numbers of bacteria in the lung and blood,significantly milder lung tissue injury,and significant increases in the numbers of leukocytes and neutrophils and the levels of TNF-α and IL-1β in BALF (P＜0.05).Conclusion Sodium new houttuyfonate can significantly increase the number and activity of leukocytes in mice with Acinetobacter baumannii pulmonary infection and thus enhance anti-infection ability.关键词
新鱼腥草素钠/鲍曼不动杆菌/肺炎/免疫缺陷Key words
Sodium new houttuyfonate/Acinetobacter baumannii/Pneumonia/Immunodeficiency分类
医药卫生引用本文复制引用
张怡敏,周雪宁,张宏方,环诚,叶峥嵘..新鱼腥草素钠对小鼠鲍曼不动杆菌肺感染模型的保护作用[J].安徽中医药大学学报,2018,37(1):67-71,5.基金项目
陕西中医药大学自然科学青年基金项目(2016QN29) （2016QN29）
