北京中医药大学学报2018，Vol.41Issue(4)：294-300,7.DOI:10.3969/j.issn.1006-2157.2018.04.006
丹参配方颗粒及丹参超细粉抗脑缺血再灌注损伤作用的比较研究
Protective effects of concentrate granule and ultrafine powder of danshen on cerebral ischemia-reperfusion injury: a comparative study
摘要
Abstract
Objective To compare the protective effects of concentrate granule and ultrafine powder of danshen (Salvia Miltiorrhizae Radix et Rhizome) on cerebral ischemia-reperfusion (CIR) injury. Methods Male ICR mice were randomly divided into sham operation group,model group,0.45 g/(kg ·d) danshen concentrate granule group and 2.25 g/(kg·d) danshen ultrafine powder group. CIR model was established by using middle cerebral artery occlusion(MCAO). Weight of mice was monitored throughout the experiment to evaluate their health status. The influence on neurological injury,movement and sensory function was evaluated by using neurologic score test,corner test and grip strength test. The effects of ultrafine powder and concentrate granule on CIR injury were compared using the following parameters:content of water in brain,infraction volume with TTC staining, and tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and myeloperoxidase (MPO) in the brain by using ELISA.Results Compared with CIR model group,the grip strength and body weight improved significantly (P<0.01 or P<0.05) and contents of TNF-α and MPO decreased markedly (P<0.01 or P<0.05) in ultrafine powder group; content of water in brain decreased significantly (P <0.05) in concentrate granule group;cerebral infarction volume decreased markedly (P<0.01) in both danshen groups(P<0.01). Conclusion The danshen concentrate granule and ultrafine powder both have a protective effect on ischemia-reperfusion injury,and their efficacy is similar at the same dosage.关键词
丹参配方颗粒/丹参超细粉/脑缺血/再灌注/炎症因子/小鼠Key words
concentrate granule of Salvia Miltiorrhizae Radix et Rhizome/ultrafine powder of Salvia Milti-orrhizae Radix et Rhizome/cerebral ischemia/reperfusion/inflammatory cytokines/mice分类
医药卫生引用本文复制引用
李亚,高文慧,唐民科..丹参配方颗粒及丹参超细粉抗脑缺血再灌注损伤作用的比较研究[J].北京中医药大学学报,2018,41(4):294-300,7.基金项目
重大新药创制国家科技重大专项(No.2011ZX09201-201-22),北京中医药大学研究生自主课题项目(No.2017-JYB-XS-065)National Science and Technology Major Projects for"Major New Drugs Innovation and Development"(No.2011ZX09201-201-22) （No.2011ZX09201-201-22）
