北京中医药大学学报2018，Vol.41Issue(4)：301-305,5.DOI:10.3969/j.issn.1006-2157.2018.04.007
黑果枸杞对H2O2诱导的H9c2心肌细胞氧化应激损伤的保护作用研究
Protective effects of Lycium ruthenicum Murr. on hydrogen peroxide-induced cellular oxidative stress injury in heart-derived H9c2 cells
摘要
Abstract
Objective To explore the protective effects of Lycium ruthenicum Murr. (Lrm) on hydrogen peroxide (H2O2)-induced cellular oxidative stress injury in heart-derived H9c2 cells. Methods In this study,H2O2-induced injury in heart-derived H9c2 cells was established as cellular oxidative stress injury model. The H9c2 cells were divided into blank group,model group(H2O2400 μmol/L) and experimental group(Lrm 1.00 g/L +H2O2400 μmol/L). The Lrm group was pretreated with Lrm for 2 h before treated with H2O2for 6 h. Cellular morphology of all groups was observed. Cell viability(MTT assay),apoptosis rate (TUNEL assay),Bcl-2 and Bcl-2 relating x gene protein (Bax) expression were evaluated (Western Blotting). Results Compared with the blank group, abnormal cellular morphology, decreased cell activity,and increased apoptosis were observed in the model group(P<0.05). Anti-apoptotic Bcl-2 gene was less expressed while pro-apoptotic Bax protein level was increased. Compared with the model group, cellular morphology and activity was greatly improved in the experimental group with reduced apoptosis rate (P <0.05). The increased expression of Bcl-2 and reduction of Bax were also significantly different. Conclusion Our findings suggest that Lrm tended to improve the H9c2 myocardial cellular morphology, enhance cell activity and reduce apoptosis rate. Its mechanism of action in reducing oxidative stress injury may be related to the up-regulation of Bcl-2 as well as down-regulation of Bax protein expression.关键词
黑果枸杞/氧化应激损伤/凋亡/B淋巴细胞瘤-2/B淋巴细胞瘤-2相关x基因Key words
Lycium ruthenicum Murr./oxidative damage/apoptosis/Bcl-2/Bax分类
医药卫生引用本文复制引用
孙慧娟,董玲,王嘉馨,潘孟,武越,李森..黑果枸杞对H2O2诱导的H9c2心肌细胞氧化应激损伤的保护作用研究[J].北京中医药大学学报,2018,41(4):301-305,5.基金项目
国家中药标准化项目(No.ZYBZH-Y-GS-10-B),国家自然科学基金青年科学基金项目(No.81703942),北京中医药大学科研发展基金项目(No.2017-HXF2JJ-006)National Standardization Project of Traditional Chinese Medicine(No.ZYBZH-Y-GS-10-B),National Natural Youth Science Foundation of China(No.81703942) （No.ZYBZH-Y-GS-10-B）
