北京中医药大学学报2018，Vol.41Issue(4)：306-309,4.DOI:10.3969/j.issn.1006-2157.2018.04.008
枳葛解酒保肝方对酒精性肝损伤大鼠SOD、MDA、GSH的影响
Effects of Zhige Jiejiu Baogan Fang on SOD,MDA and GSH in rats with alcoholic liver injury
摘要
Abstract
Objective To investigate the protective effects of Zhige Jiejiu Baogan Fang(Semen Hoveniae and Radix Puerariae Lobatae hangover-relieving and liver-protecting recipe, ZGJJBGF) on superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH) rats with alcoholic liver injury. Methods 180 male Wistar rats were randomly divided into 6 groups: blank group, model group, positive control (Compound Methionine and Choline Bitartrate Tablet) group (0.36 g/kg), high-dose, mid-dose and low-dose ZGJJBGF groups (18,9,1.8 g/kg respectively). Except for the blank group, rats in other groups were orally administered with 50% ethanol(diluted with distilled water) for 6 weeks to establish the alcoholic liver injury rat model. Rats in all experimental groups except the blank and model groups received medicinal intervention respectively. The activity of malondialdehyde (MDA), glutathione (GSH),and superoxide dismutase(SOD) in serum and liver were measured at the end of the experiment. Results SOD activity in model group was significantly lower than that in the blank group, and the contents of MDA and GSH in the liver tissues and blood were significantly higher in the blank group (P<0.01). Compared with model group, mid-dose ZGJJBGF significantly increased the serum GSH level (P<0.05) and the activity of SOD (P<0.01) while reducing the content of MDA in liver tissue(P<0.01). Compared with the model group,high-dose ZGJJBGF significantly reduced the content of MDA in liver tissue of rats(P<0.01) and increased the content of GSH(P<0.01). Compared with model group, low-dose ZGJJBGF reduced serum MDA content (P<0.01). Conclusion Zhige Jiejiu Baogan Fang could enhance the antioxidant ability of endogenous antioxidant enzymes such as SOD and GSH. It can also inhibit the damage of liver injury of free radical-induced lipid peroxidation.关键词
枳葛解酒保肝方/酒精性肝损伤/超氧化物歧化酶/丙二醛/还原型谷胱甘肽/大鼠Key words
Zhige hangover-relieving and liver-protecting recipe/alcoholic liver injury/superoxide dis-mutase/malondialdehyde/reduced glutathione/rats分类
医药卫生引用本文复制引用
陈丰,刘殿娜,陈绍红,柳海艳,修琳琳,高洁,李娜,张晨,钟赣生..枳葛解酒保肝方对酒精性肝损伤大鼠SOD、MDA、GSH的影响[J].北京中医药大学学报,2018,41(4):306-309,4.基金项目
国家自然科学基金项目(No.81274104),北京中医药大学科研发展基金项目(No.2010072120035)National Natural Science Foundation of China(No.81274104) （No.81274104）
