北京中医药大学学报2018，Vol.41Issue(4)：316-328,13.DOI:10.3969/j.issn.1006-2157.2018.04.010
基于药物体系酚类特征架构的决明子质量表征关联分析研究
Quality representation and correlation analytical study of Semen Cassiae based on phenolic characteristic architecture of medical system
摘要
Abstract
Objective To establish the quality representation and correlation analysis method and the content of characteristic ingredients based on spectrum of medicinal system of Juemingzi (Semen Cassiae),in order to evaluate the quality of Semen Cassiae decocting pieces effectively and accurately. Methods HPLC-PDA method was established to characterize the quality of spectrum of Semen Cassiae medicinal system architecture;quality of 11 batches of Semen Cassiae decocting pieces was characterized based on the number and chemical type of peaks on characteristic spectrum. The content of 6 characteristic index components, naphthopyrones and anthraquinones were characterized by indexes in characteristic spectrum. Correlation analysis of quality of Semen Cassiae was then performed with reference to the standard reference pieces. Results Taken as the reference substance, characteristic spectrum of batch 10 Semen Cassiae altogether contains 12 characteristic peaks, all characteristic peaks appeared on the chromatograms of 11 batches of Semen Cassiae. In 11 batches of Semen Cassiae, No.5, 11, 8, 1, 2 pieces contained higher effective index components, while No. 5, 11, 2, 9, and 7 pieces had high relevance with the reference piece (No. 10). Consolidated quality characterization and correlation analysis,the quality of No. 5,11,8,1,2,9,and 7 were excellent and dominant. Conclusion The HPLC method to analyze quality and quantity of phenolic components spectrum of 11 batches of Semen Cassiae is simple and accurate. The evaluation mode of quantity and quality and their correalation based on Semen Cassiae medical system, including systemic correlation and appilication validity, as it can provide a reliable methodological basis for the comprehensive quality evaluation of Semen Cassiae.关键词
决明子/酚类/特征图谱/关联分析/质量评价Key words
Semen Cassiae/phenolic/characteristic spectrum/correlation analysis/quality evaluation分类
医药卫生引用本文复制引用
刘璐,李文霞,李思潼,范圆圆,黄玉娟,冯鑫,石任兵,李倩,周德勇,孙道涵,路东波,孔静,才秀芬,王梦林,武秋红..基于药物体系酚类特征架构的决明子质量表征关联分析研究[J].北京中医药大学学报,2018,41(4):316-328,13.基金项目
十二五国家科技支撑计划项目(No.2012BAI29B06),北京市教委共建科研基地建设项目,北京中医药大学重点学科开放课题(No.2013-2DXKKF-23)National Sci-Tech Plan of the 12th Five-Year Plan(No.2012BAI29B06),Scientific Projects of Beijing Municipal Commission of Education （No.2012BAI29B06）
