波谱学杂志2018，Vol.35Issue(1)：8-21,14.DOI:10.11938/cjmr20172576
基于NMR的4T1荷瘤小鼠脾脏受不同给药方式影响的代谢组学研究
NMR-Based Metabonomic Analyses on Spleen Tissues of 4T1 Tumor-Bearing Mice Subjected to Chemotherapies with Different Drug Delivery Strategies
摘要
Abstract
The combination of doxorubicin and paclitaxel is widely used in clinical cancer therapy.Due to enhanced permeation and retention effects of nanomaterials in tumor tissues,nanoearriers with good biocompatibility have been designed to encapsulate doxorubicin/paclitaxel,and used to facilitate delivery of the drugs to achieve better therapeutic outcome.In this study,NMR-based metabonomic analyses were used to study the metabolic profiles in spleen tissues of normal healthy mice and of 4T1 tumor-bearing mice,which were treated with either saline,or doxorubicin/paclitaxel in their free forms,or doxorubicin/paclitaxel encapsulated in nanocarriers.The results demonstrated that treatment with the free forms of doxorubicin/paclitaxel remitted tumor-induced disturbances in gut mierobiota,while treatment with nanoearrier-encapsulated doxorubicir/paclitaxel alleviated increased glucose and fumarate metabolism associated with tumor bearing.In contrast,tumor-related splenomegaly and metabolic alterations in amino acids,organic acids,nucleic acids and choline,both attributable to immune responses of the spleen,were not ameliorated by drug treatment.This could also be due to the fact that the treatment period was too short to have any significant effects.This work provided metabolic phenotypical information of the spleen tissues in 4T 1 tumor-bearing mice,and helped to understand the metabolic impacts of drug delivery systems on the spleen.关键词
阿霉素/紫杉醇/纳米载体/脾脏/代谢组学Key words
doxorubicin/paclitaxel/nanocarrier/spleen/metabonomics分类
数理科学引用本文复制引用
胡依黎,豪富华,王玉兰..基于NMR的4T1荷瘤小鼠脾脏受不同给药方式影响的代谢组学研究[J].波谱学杂志,2018,35(1):8-21,14.基金项目
国家重点基础研究发展计划(“973计划”)资助项目(2012CB934004). （“973计划”）
