草业学报2018，Vol.27Issue(2)：117-123,7.DOI:10.11686/cyxb2017317
细叶百合LpAGD14基因的克隆与表达分析
Cloning and expression analysis of LpAGD14 from Lilium pumilum
摘要
Abstract
A gene encoding an ArfGAP protein was cloned from the cDNA library of Lilium pumilum.Its ORF sequence encoded a 640-amino acid polypeptide with a typical C4 ArfGAP conserved domain.The relative molecular mass of the putative ArfGAP protein was 69.43 kDa,the theoretical isoelectric point was 8.95,and it was predicted to be a hydrophilic protein with no transmembrane structure.At the amino acid sequence level,the L.purnilum ArfGAP showed 59％ homology to ArfGAP-domain proteins (AGDs) in Elaeis guineensis,and more than 50％ homology to AGDs in Musa acuminata subsp.,Phoenix dactylifera,and Ananas comosus.Therefore,the gene was named LpAGD14.Transcript analysis by qRT-PCR showed that the transcript levels of LpAGD14 increased to reach the highest value after dormancy release.Subcellular localization assays showed that LpAGD14 localized to the cell membrane.Ultrastructural observations showed that the layers of the Golgi body increased with prolonged cold storage.These results indicated that LpAGD14 is involved in the dormancy release of L.pumilum and plays a role in vesicle transport.This study lays the foundation for further research on the function and regulation of this gene and its product.关键词
细叶百合/ArfGAP/克隆/表达Key words
Lilium pumilum/ArfGAP/cloning/expression引用本文复制引用
汪王,田忠平,苏小霞,杨柳慧,周蕴薇..细叶百合LpAGD14基因的克隆与表达分析[J].草业学报,2018,27(2):117-123,7.基金项目
国家自然科学基金项目(31470698)资助. （31470698）
