重庆医学2018，Vol.47Issue(10)：1301-1304,4.DOI:10.3969/j.issn.1671-8348.2018.10.002
微小RNA-134-5p靶向作用于EGFR对卵巢癌SKOV3和A2780细胞生长的影响
Effect of microRNA-134-5p targeting EGFR on growth of ovarian cancer SKOV3 and A2780 cells
摘要
Abstract
Objective To investigate the effect of microRNA-134-5p (miR-134-5p) targeting epidermal growth factor receptor (EGFR) on the growth of ovarian cancer cells.Methods The ovarian cancer cell lines SKOV3 and A2780 served as the study objects and were divided into the control group (transfecting miR-NC) and experimental group (transfecting miR-134-5p) according to the treatment method.The expression levels of EGFR gene and downstream target protein were detected by qRT-PCR and western blot.The cell cycle distribution and apoptosis were detected by flow cytometry.The proliferation ability of ovarian cancer cells was detected by MTT assay and colony forming assay.Results The expressions of EGFR and downstream target protein in the experimental group were significantly down-regulated.EGFR mRNA in SKOV3 cells was downregulated to 48％ (P＜0.05),and EGFR mRNA in A2780 cells was down-regulated to 47％ (P＜0.05).The cell cycle of cells in the experimental group was significantly inhibited (P＜0.05),and miR-134-5p induced apoptosis through the EGFR target protein (P＜0.05).The proliferation activity and colony forming ability of the experimental group were significantly inhibited (P＜0.05).Conclusion miR-134-5p could promote the cellular cycle arrest and apoptosis,and reduces the proliferation ability of ovarian cancer cells by targetedly inhibiting the EGFR gene.关键词
微RNAs/卵巢肿瘤/基因,肿瘤抑制/受体,表皮生长因子/SKOV3/A2780Key words
microRNAs/ovarian neoplasms/genes, tumor suppressor/receptor, epidermal growth factor/SKOV3/A2780分类
医药卫生引用本文复制引用
汤继英,陈萍,蔡晓军,汪选斌,曹风军,张莉..微小RNA-134-5p靶向作用于EGFR对卵巢癌SKOV3和A2780细胞生长的影响[J].重庆医学,2018,47(10):1301-1304,4.基金项目
湖北省教育厅科学技术研究项目(B2016139) （B2016139）
湖北省高校优秀中青年创新基金项目(T201510). （T201510）
