重庆医学2018，Vol.47Issue(10)：1314-1317,4.DOI:10.3969/j.issn.1671-8348.2018.10.005
原花青素对脑出血后脑水肿及继发性脑损伤炎症的作用研究
Effects of proanthocyanidins on cerebral edema and secondary brain injury inflammation following intracerebral hemorrhage
摘要
Abstract
Objective To investigate the effect of procyanidins on cerebral edema and inflammation of secondary brain injury after intracerebral hemorrhage.Methods Forty-eight C57BL6 mice were randomly divided into 4 groups:sham-operation+ saline group,sham-operation + procyanidins (100 mg/kg) group,intracerebral hemorrhage (ICH) + saline group and ICH + procyanidins (100 mg/kg) group.The Bederson method was used to detect postoperative nerve function score in mice.The brain tissue water content was measured by wet-dry weight method.The variety of morphological in brain tissue was observed by hematoxylin-eosin (HE) staining.The expression of glial fibrillary acidic protein (GFAP) and ionized calcium-binding adapter molecule 1(Iba-1) in mouse brain tissue were observed by western blot.The apoptosis of brain tissue was observed by TUNEL and western blot method.Results Compared with the ICH+saline group after 12h,the nerve function score in the ICH + proanthocyanidins group was significantly decreased (P＜0.05),and the water content of brain tissue was also significantly decreased (P＜0.05).The HE staining results showed that procyanidins decreased the number of neurocytes lesions in cerebral hemorrhage mice and reduced the amount of brain tissue hemorrhage.Western blot results showed that procyanidins promoted the expression of Bcl-2,decreased the expression of Bax,Caspase3,GFAP and Iba-1 in cerebral hemorrhage mice.The TUNEL results showed that procyanidins could inhibit the apoptosis of brain tissue neurons in mouse with intracerebral hemorrhage.Conclusion Procyanidins inhibits the production of neurotoxic substances possibly by reducing the expression of GFAP and Iba-1,and prevents and treats secondary brain injury after intracerebral hemorrhage.关键词
原花青素类/脑出血/继发性脑损伤Key words
procyanidins/cerebral hemorrhage/secondary brain injury分类
医药卫生引用本文复制引用
石磊,赵焱,徐治强,王建祥,彭翔..原花青素对脑出血后脑水肿及继发性脑损伤炎症的作用研究[J].重庆医学,2018,47(10):1314-1317,4.基金项目
武汉市卫生和计划生育委员会科研项目(WZ16C17). （WZ16C17）
