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滇中茶树种质资源亲缘关系的ISSR分析

吴田 蓝增全 王华芳

福建农业学报2017,Vol.32Issue(12):1298-1302,5.
福建农业学报2017,Vol.32Issue(12):1298-1302,5.DOI:10.19303/j.issn.1008-0384.2017.012.005

滇中茶树种质资源亲缘关系的ISSR分析

ISSR-based Genetic Analysis on Relationship of Teas in Central Yunnan

吴田 1蓝增全 2王华芳3

作者信息

  • 1. 西南林业大学园林学院,云南昆明650224
  • 2. 西南林业大学环境科学与工程学院,云南 昆明 650224
  • 3. 北京林业大学生物科学与技术学院,北京 100083
  • 折叠

摘要

Abstract

The genetic relationship among 17 representative tea cultivars from various regions in central Yunnan was analyzed using ISSR markers.Out of the randomly selected 28 primers in testing DNA polymorphism of the teas,15 produced distinctive bands and were chosen for amplification.The ISSR-PCR amplified primers showed 112 stable and clearly defined bands.Of which,103 were polymorphic (a rate of 91.96%) averaging 7.4 bands each.The GS scores on the cultivars ranged from 0.58 to 0.80 with discrete classifications of Group I that consisted of 12 germplasms and Group Ⅱ that composed of the remaining 5 germplasms.

关键词

宝洪茶/十里香茶/ISSR/亲缘关系

Key words

central Yunnan/tea cultivars/ISSR-PCR/genetic relationship

分类

农业科技

引用本文复制引用

吴田,蓝增全,王华芳..滇中茶树种质资源亲缘关系的ISSR分析[J].福建农业学报,2017,32(12):1298-1302,5.

基金项目

中西部高等学校青年骨干教师国内访问学者项目(2016-2017) (2016-2017)

澜沧江流域古茶研究项目(2017) (2017)

昌宁县茶树资源保护利用项目(2017) (2017)

福建农业学报

OA北大核心CSTPCD

1008-0384

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