福建农业学报2017,Vol.32Issue(12):1298-1302,5.DOI:10.19303/j.issn.1008-0384.2017.012.005
滇中茶树种质资源亲缘关系的ISSR分析
ISSR-based Genetic Analysis on Relationship of Teas in Central Yunnan
摘要
Abstract
The genetic relationship among 17 representative tea cultivars from various regions in central Yunnan was analyzed using ISSR markers.Out of the randomly selected 28 primers in testing DNA polymorphism of the teas,15 produced distinctive bands and were chosen for amplification.The ISSR-PCR amplified primers showed 112 stable and clearly defined bands.Of which,103 were polymorphic (a rate of 91.96%) averaging 7.4 bands each.The GS scores on the cultivars ranged from 0.58 to 0.80 with discrete classifications of Group I that consisted of 12 germplasms and Group Ⅱ that composed of the remaining 5 germplasms.关键词
宝洪茶/十里香茶/ISSR/亲缘关系Key words
central Yunnan/tea cultivars/ISSR-PCR/genetic relationship分类
农业科技引用本文复制引用
吴田,蓝增全,王华芳..滇中茶树种质资源亲缘关系的ISSR分析[J].福建农业学报,2017,32(12):1298-1302,5.基金项目
中西部高等学校青年骨干教师国内访问学者项目(2016-2017) (2016-2017)
澜沧江流域古茶研究项目(2017) (2017)
昌宁县茶树资源保护利用项目(2017) (2017)