福建农业学报2018，Vol.33Issue(1)：1-6,6.DOI:10.19303/j.issn.1008-0384.2018.01.001
CpG-ODN2006对斑马鱼抗创伤弧菌感染的作用
Functions of CpG-ODN 2006 on Resistance of Zebra Fish against Vibrio vulnificus
摘要
Abstract
Groups of zebra fish were injected with either PBS as controlor synthetic CpG-ODN 2006 for treatment,and 48 hours later,challenged by Vibrio vulnificus FJ03-X2 infection in this study.The immune protection of the treatment group reached a rate of 70.0％ in 24 hours.RNA was extracted from the intestinal and kidney tissues and then reverse transcribed into cDNA.The mRNAs of lysozyme,Myd88,tlrs and tnf in the guts of the treated fish were significantly downregulated,while those of il1b and il10,significantly upregulated,as detected by Real-time RT-PCR.The differences on gene expression in kidney were even more apparent as the expression of lysozyme significantly upregulated,and those of il1b,myd88,tnf,ifng1-2,il10 and il22 significantly downregulated.It appeared that the CpG-ODN 2006 injection afforded the zebra fish to vitalize its immune system through regulating the expressions of non-specific immunity-related genes in the kidney and intestinesto ward off the viral attack.关键词
斑马鱼/CpG-ODN/创伤弧菌/非特异性免疫Key words
zebra fish/CpG-ODNs/Vibrio vulnificus/non-specific immunity分类
农业科技引用本文复制引用
陈华,李素一,张丽娟,柯翎,陈叙,林晨韬..CpG-ODN2006对斑马鱼抗创伤弧菌感染的作用[J].福建农业学报,2018,33(1):1-6,6.基金项目
福建省科技计划项目——省属公益类科研院所基本科研专项(2015R1019-10、2016R1018-6、2016R1018-3) （2015R1019-10、2016R1018-6、2016R1018-3）
福建省农业科学院青年人才创新基金项目(2014QB-29) （2014QB-29）
福建省自然科学基金项目(2015J01104) （2015J01104）
福建省农业科学院科技创新团队PI项目(2016PI-22) （2016PI-22）
国家自然科学基金项目(31100658、31300766) （31100658、31300766）
福建省农业科学院青年英才计划项目(YC2015-20) （YC2015-20）
