福建农业学报2018，Vol.33Issue(1)：12-16,5.DOI:10.19303/j.issn.1008-0384.2018.01.003
鳗鲡圆环病毒PCR检测方法的建立及应用
A PCR Methodology for Detecting Eel Circovirus
李苗苗1
作者信息
- 1. 福建省水产技术推广总站,福建福州 350002
- 折叠
摘要
Abstract
Eel circovirus (EeCV) is one of the pathogens that cause virosis on eels.Based on the gene sequence of EeCV in GenBank,a pair of primers was selected for the development of the detection method using PCR.The optimized reaction conditions,as well as the specificity,sensitivity and repeatability of the methodology were determined to establish the preliminary testing protocol.Subsequently,160 epidemic specimens with the suspected viral infection were collected from are as in Fujian for the determination.Out of all samples,65 were shown to be positive,at a rate of 40.6％.It appeared that the current PCR method could be employed to detect EeCV for early disease diagnosis on eels.关键词
鳗鲡圆环病毒(EeCV)/PCR检测/方法建立/应用Key words
eel circovirus/PCR detection/detection methodology/application分类
农业科技引用本文复制引用
李苗苗..鳗鲡圆环病毒PCR检测方法的建立及应用[J].福建农业学报,2018,33(1):12-16,5.
