福建农业学报2018，Vol.33Issue(1)：29-34,6.DOI:10.19303/j.issn.1008-0384.2018.01.006
基于EST-SSR的64个茶树品种遗传多样性与群体结构分析
EST-SSR-based Analyses on Genetic Diversity and Population Structure of 64 Tea Varieties
摘要
Abstract
To establish a systematic classification for the tea varieties in Fujian,Zhejiang and Guangdong,20 pairs of SSR primers were selected in analyzing the genetic diversity and population structure of 64 tea cultivars commonly found in the regions.A total of 147 polymorphic loci were amplified with an average of 7.35 alleles per primer (ranging 3-15) and an average polymorphism information contentof 0.588 (ranged 0.267-0.851).The expected heterozygosity varied from 0.247 to 0.838 averaging 0.559;while,the observed heterozygosity,from 0.308 to 0.870 averaging 0.636.The Shannon index of the 64 teas was 1.271.The maximum ad hoe quantity,AK,was observed when K=4 in the population structure analysis,indicating that the entire collection could be divided into four subpopulations.Using a membership probability threshold of ≥0.50,60 genotypes (accounting for 93.8％ of the tested genotypes) were assigned to the four subpopulations,and 4 (accounting for 6.2％ of the tested breeding parents) retained in the admixed group.关键词
茶树/EST-SSR/遗传多样性/群体结构Key words
tea plant/EST-SSR/genetic diversity/population structure分类
农业科技引用本文复制引用
杨军,王让剑,孔祥瑞,郑国华,苏峰..基于EST-SSR的64个茶树品种遗传多样性与群体结构分析[J].福建农业学报,2018,33(1):29-34,6.基金项目
福建省现代农业(茶叶)产业技术体系建设专项(2014-2017年) （茶叶）
福建省科技计划项目——省属公益类科研院所基本科研专项(2015R1012-7、2017R1012-6) （2015R1012-7、2017R1012-6）
福建省农业科学院科技创新团队建设项目(STIT2017-3-12) （STIT2017-3-12）
