福建农业学报2018，Vol.33Issue(1)：54-60,7.DOI:10.19303/j.issn.1008-0384.2018.01.011
小菜蛾饲料品系转食寄主植物的适合度及其硫苷代谢相关基因表达
Fitness and Glucosinolate Catabolism-related Gene Expression Upon Feeding on Natural Host Plant of Plutella xylostella Raised from Artificial Diet
摘要
Abstract
Glucosinolate sulfatase (GSS) and sulfatase modifying factor 1 (SUMF1) are crucial for the catabolism of Plutella xylostella on the defensive glucosinolates in the cruciferous host plants.However,little information is available on their roles in the adaptation of the strain of P.xylostella raised from an artificial diet when changed to be fed on its natural host plant.The expression patterns of GSSs and SUMF1s at different developmental stages of two artificial diet strains of P.xylostella (i.e.,AD and G88) were determined.It was found that the expressions of GSSs were similar between the two strains,with abundant GSS1 and GSS2 expressions at the 3rd and 4th-instar stages,but no apparent patterns observed for SUMF1s.After the newly hatched larvae of AD and G88 were transferred onto the cotyledons of radish plants,the larval survival rates became lower,with longer larval developmental time and lower pupal weight,than their counterparts fed on the original artificial diet.The expression levels of GSS1 and GSS2 in the larval midguts grown on the radish cotyledons decreased significantly;but,that of SUMF1a,only in the midguts of AD strain.It suggested that the expressions of glucosinolate catabolism-related genes in P.xylostella were possibly regulated by the factor (s) in the host plant and closely associated with the adaptability of the insects upon a shifted feeding from a formulated diet to a natural host plant.关键词
小菜蛾/硫代葡萄糖苷硫酸酯酶/硫酸酯酶修饰因子/人工饲料品系/转寄主Key words
Plutella xylostella/glucosinolate sulfatase/sulfatase modifying factor 1/artificial diet strain/host shift分类
生物科学引用本文复制引用
董玉红,陈玮,郑玲,荆晓东,周立,张玲玲,李晓桐,何玮毅..小菜蛾饲料品系转食寄主植物的适合度及其硫苷代谢相关基因表达[J].福建农业学报,2018,33(1):54-60,7.基金项目
国家重点研发计划(2017YFD0200400) （2017YFD0200400）
福建省自然科学基金项目(2014J01086) （2014J01086）
福建农林大学“校杰出青年科研人才”项目(xjq201403) （xjq201403）
