甘蔗糖业Issue(2)：1-5,5.
rDNA在斑茅染色体的物理定位和染色体基数研究
Physical Mapping of rDNA on the Chromosomes of Erianthus Arundinaceus and Determination of Basic Chromosome Number
摘要
Abstract
In order to know the physical mapping of 5S rDNA and 45S rDNA on the chromosomes of E. arundinaceus and determination of basic chromosome number,the E.arundinaceus chromosome localization of 5S rDNA and 45S rDNA was studied by fluorescence in situ hybridization on metaphase chromosomes in Hainan 92-77 (2n=60) and Hainan 92-105 (2n=60). The results showed that the number of 45S rDNA site signal loci were six at somatic chromosomes of metaphase and cell nucleus of interphase in both Hainan 92-77 and Hainan 92-105, and the signal loci of 45S rDNA was located in the short arm end of six chromosomes. Among them, loci were different, suggesting that the copy number of 45S rDNA was different. The number of 5S rDNA site signal loci were six in both Hainan 92-77 and Hainan 92-105, and the signal loci of 45S rDNA was located near the long arm of chromosome centromere, all the 6 loci were of similar size, suggesting that the copy number of 5S rDNA was similar.Therefore,the basic chromosome number of E.arundinaceus with 2n=60 was determined as x=10.关键词
斑茅/荧光原位杂交/核糖体DNA/物理定位/染色体基数Key words
Erianthus arundinaceus/Fluorescence in situ hybridization/Ribosomal DNA/Physical mapping/Basic chromosome number分类
农业科技引用本文复制引用
吴嘉云,黄永吉,王勤南,凌秋平,邓祖湖,李奇伟,陈如凯..rDNA在斑茅染色体的物理定位和染色体基数研究[J].甘蔗糖业,2018,(2):1-5,5.基金项目
广东省基金项目(2015A030310286) （2015A030310286）
国家基金项目(31571730、31701488、31401440) （31571730、31701488、31401440）
广东省科技计划项目(2014A030304027、2015A030302030、2017A030303048) （2014A030304027、2015A030302030、2017A030303048）
广东省科学院实施创新驱动发展能力建设专项(2017GDASCX-0105) （2017GDASCX-0105）
