环境保护科学2018，Vol.44Issue(2)：65-70,6.DOI:10.16803/j.cnki.issn.1004-6216.2018.02.013
甲醛降解功能菌的分离鉴定及降解特性
Isolation and Identification of Bacteria Strains Able to Degrade Formaldehyde and their Degrading Characteristics
摘要
Abstract
Bacteria strains capable to degrade and metabolize formaldehyde were isolated from the ceramsite covered with biofilm in the packing tower by taking formaldehyde as a sole carbon source and energy, with the aim at providing strain sources for the further study of the mechanism of formaldehyde biodegradation. Environmental microbial analysis, physiological and biochemical tests, and 16S rDNA gene sequence analysis were used to identify and classify the strains. Single factor experiment was adopted to evaluate the effects of various parameters including pH, biomass, concentration of formaldehyde on formaldehyde degradation and hang cell membranes onto the screened strains to establish a new packing tower system. Two selected strains were named P1and Q1and isolated and identified as Pseudomonas sp. and Methylobacterium sp. respectively. When the concentration of formaldehyde in the culture medium was 300 mg/L, under the same cultural conditions, 92.8% of formaldehyde was degraded by strain P1within 44 h, and 97.9% degraded by strain Q1within 28 h.When the original concentration of formaldehyde was below 700 m/L, formaldehyde was degraded by strain P1and strain Q1completely, and strain Q1was well tolerated in a higher concentration of formaldehyde. Furthermore, the removal efficiency of formaldehyde by the two mixed bacteria strains reached to more than 99%. After 21 days, the formaldehyde removal amount remained at 16 mg/ (L·h), indicating that the packing tower grew well with a higher purification effect.关键词
甲醛/假单胞菌属/甲基营养菌属/降解特性Key words
Formaldehyde/Pseudomonas/Methylobacterium/Characteristics of Degradation分类
资源环境引用本文复制引用
张明,王洁,孙珮石,申艳萍,张艮林,邹平,毕晓伊..甲醛降解功能菌的分离鉴定及降解特性[J].环境保护科学,2018,44(2):65-70,6.基金项目
国家自然科学基金项目(51268058) （51268058）
云南大学人才引进与培养计划(C6176102)资助 （C6176102）
