河南农业科学2017，Vol.46Issue(12)：126-129,4.DOI:10.15933/j.cnki.1004-3268.2017.12.023
辽宁地区猪源大肠埃希菌喹诺酮类耐药基因的检测与分析
Detection and Analysis of Quinolone Resistance Genes in Swine Escherichia coli from Liaoning Area
摘要
Abstract
To investigate master distribution of plasmid-mediated quinolone resistance genes (PMQR),quinolone resistant determining regions(QRDRs) mutation characteristics of DNA helicase genes (gyrA,gyrB) and topoisomerase Ⅳ genes (parC,parE)and their effects on the bacterial resistance to the quinolone antibiotics of Escherichia coli from Liaoning area.gyrA,gyrB,parC,parE,aac (6')-Ib-cr,qepA,qnrA,qnrB,qnrC,qnrD and qnrS genes of 23 quinolone resistant strains isolated were detected by PCR.The results showed that positive rate of PMQR gene was 86.96％(20/23),in addition 5 isolates harbored three kinds of PMQR genes,9 isolates harbored two kinds of PMQR genes and 6 isolates harbored one kinds of PMQR genes.Positive rate of aac(6')-Ib-cr gene was the highest,followed by qepA,qnrB,qnrC and qnrS genes,none of qnrA and qnrD genes was detected;Analysis of gyr and par genes QRDRs mutation among 15 resistant strains were isolated,mutation rate of K447E was the highest,followed by D87N,D426N,S80I and A56T.Therefore,PMQR genes and chromosome mediated target mutation is important cause of resistance to quinolones for Escherichia coli from swine in Liaoning area.关键词
猪源大肠埃希菌/喹诺酮类耐药基因/PCR/序列分析/辽宁Key words
swine Escherichia coli/quinolone resistance genes/PCR/sequential analysis/Liaoning分类
农业科技引用本文复制引用
赵凤菊,关淼,李清竹,顾贵波..辽宁地区猪源大肠埃希菌喹诺酮类耐药基因的检测与分析[J].河南农业科学,2017,46(12):126-129,4.基金项目
辽宁省自然科学基金项目(201402573) （201402573）
