河南农业科学2017，Vol.46Issue(12)：139-143,5.DOI:10.15933/j.cnki.1004-3268.2017.12.026
青麦仁种皮膳食纤维的提取及其抗氧化活性研究
Extraction Methods and Resistance to Oxidation of Dietary Fiber from Testa of the Green Wheat Kernel
摘要
Abstract
In this study,the antioxidant activity of dietary fiber prepared from the seed coat of fresh green wheat was evaluated by measuring the content of total flavonoids,DPPH free radical scavenging ability,reducing ability and hydroxyl free radical scavenging capacity.The optimal extraction methods of water-soluble dietary fiber and insoluble dietary fiber of green wheat seed-coat were determined by comparative analysis of four extraction methods of enzyme-alkali method,double enzyme method,ultrasound-assisted enzyme method and microwave assisted enzyme method.The results showed that the ultrasound-assisted enzyme method had better effect on improving the antioxidant activity of the water-soluble dietary fiber.The content of total flavonoids in soluble dietary fiber was 1.486 mg/g.The scavenging ability of DPPH radical was 76.85％.The scavenging ability of hydroxyl radical was 17.96％.Reducing ability (absorbance) was 0.312.The double enzyme method had better effect on improving the antioxidant activity of the insoluble dietary fiber.The content of total flavonoids in soluble dietary fiber was 2.467 mg/g.The scavenging ability of DPPH radical was 78.5％.The scavenging of hydroxyl radical was 59.80％.Reducing ability ability (absorbance) was 0.514.关键词
青麦仁/水溶性膳食纤维/不溶性膳食纤维/黄酮/抗氧化性Key words
green wheat kernel/water-soluble dietary fiber/insoluble dietary fiber/flavonoids/antioxidant分类
农业科技引用本文复制引用
张康逸,温青玉,王继红,高玲玲,康志敏..青麦仁种皮膳食纤维的提取及其抗氧化活性研究[J].河南农业科学,2017,46(12):139-143,5.基金项目
河南省重大科技专项(151100111300) （151100111300）
河南省科技开放合作项目(152106000054) （152106000054）
