湖南农业大学学报（自然科学版）2018，Vol.44Issue(2)：145-150,6.DOI:10.13331/j.cnki.jhau.2018.02.006
菜豆晕疫病原菌甘油激酶基因敲除突变体的构建与表型分析
Construction of glycerol kinase gene deletion mutants of Pseudomonas syringae pv.phaseolicola and its phenotype analysis
摘要
Abstract
GK coding sequence of Pseudomonas syringae pv.phaseolicola strain NPS3121 was cloned by PCR with the primers designed according to reported 1448A glycerol kinase (GK) amino acid sequence in GenBank. Sequencing analysis showed that the length of the GK gene was 1 506 bp,which could encode a polypeptide containing 501 amino acids. Bio–informatics analysis indicated that GK of strain NPS3121 contained 469 amino acids, with molecular weight 55 800,enriched in alpha helix,and isoelectric point was 5.44.GK deletion mutants of strain NPS3121 were constructed by integrated mutation and the results showed that the growth rate of GK deletion mutants reduced 18% and 16%, respectively in M9 and KBM medium, compared with wild strain. In addition, the content of glycerol in GK deletion mutants increased 6 times, compared to the wild strain.关键词
菜豆晕疫病菌/甘油激酶基因/敲除突变体/表型分析Key words
Pseudomonas syringae pv.phaseolicola/glycerol kinase gene/deletion mutants/phenotypical analysis分类
生物科学引用本文复制引用
刘晓柱,李银凤,于志海,刘晓辉,黄名正..菜豆晕疫病原菌甘油激酶基因敲除突变体的构建与表型分析[J].湖南农业大学学报（自然科学版）,2018,44(2):145-150,6.基金项目
贵州省教育厅创新群体重大研究项目(黔教合KY字[2017]046) （黔教合KY字[2017]046）
贵州省理工学院混合教学模式课程建设项目(2017HHKC06) （2017HHKC06）
