湖南农业大学学报（自然科学版）2018，Vol.44Issue(2)：193-197,5.DOI:10.13331/j.cnki.jhau.2018.02.015
基于RNA-Seq五指山猪背最长肌基因表达差异分析
Gene differential expression analysis in longissimus dorsi of pig–back using RNA–Seq
摘要
Abstract
In order to improve the strategy of studying gene structures and mining new genes in Wuzhishan pig, transcriptome analysis of longissimus dorsi was performed at their 6– and 8–month age using high–throughput RNA sequencing. 65 683 578 and 65 464 156 clean reads were obtained for 6– and 8 month age pig respectively, of which, 20 102 and 25 719 transcripts were confirmed. Using sequence alignment in different database after function annotations, 18 206 and 18 374 genes were found out, 687 and 737 new transcripts were predicted. The new predicted alternative splices were 28 670 and 32 940 respectively. The IR model for 6 month pig was larger while the SE shear model of 8–month pig was dominant. In addition, we anlyzed all gene structures. In conclusion, Wuzhishan pig genes have extensions and many plausible novel genes exist.关键词
五指山猪/基因表达/基因结构/可变剪接/新转录本Key words
Wuzhishan pig/gene structure/alternative splicing/new transcripts分类
农业科技引用本文复制引用
孙瑞萍,魏立民,王峰,晁哲,刘海隆,郑心力,刘圈炜,黄丽丽,邢漫萍..基于RNA-Seq五指山猪背最长肌基因表达差异分析[J].湖南农业大学学报（自然科学版）,2018,44(2):193-197,5.基金项目
海南省省属科研院所技术开发研究专项(KYYS–2015–02,琼科[2016]31号) （KYYS–2015–02,琼科[2016]31号）
国家自然科学基金项目(31560628,31760646) （31560628,31760646）
海南省农业科学院农业科技创新专项(CXZX201607) （CXZX201607）
