湖南农业大学学报（自然科学版）2018，Vol.44Issue(2)：198-203,6.DOI:10.13331/j.cnki.jhau.2018.02.016
海水和淡水养殖凡纳滨对虾肠道和鳃的菌群结构分析
Microbial community structure analysis of intestine and gill of Litopenaeus vannamei in seawater and freshwater
摘要
Abstract
The high-throughput Miseq sequencing technology was used to analyze the microbiota structure in Litopenaeus vannamei larvae, intestine and gill of young Litopenaeus vannamei under the condition of seawater and fresh water. The sequence results revealed that Firmicutes and Proteobacteria dominated in the larvae, intestines and gill with great variation in abundance. In particular,Lactococcus accounted for 36.1% in larvae microbiota community,Gammaproteobacteria_unclassified 25.5%,Bacillus 13.5%,Solibacillus 7.9%,Pseudomonas 5.0% and Arthrobacter 2.5%. In microbiota in gill tissue in both seawater and freshwater,Lactococcus,Bacillus,Solibacillus,Pseudomonas and Arthrobacter were dominant. Regarding to microbiota in intestine from seawater,Photobacterium accounted for 22.9%,Vibrio 18.2%,Lactococcus 13.3%, Cellvibrio 8.0%,Shewanella 5.5%,and Bacillus 5.4%,while in intestine from freshwater Shewanella 57.4%,Lactococcus 18.8%,Bacillus 6.3%,and Vibrio less than 0.1%.These results indicated that culture conditions had less influence on the microflora structure in gill than that while had great influence in intestine.关键词
凡纳滨对虾/肠道/鳃/海水养殖/淡水养殖/菌群结构/高通量测序Key words
Litopenaeus vannamei/intestine/gill/mariculture/freshwater aquaculture/microbial community structure/high–throughput sequencing分类
农业科技引用本文复制引用
王元,周俊芳,韦信贤,马荣荣,赵姝,李新苍,房文红..海水和淡水养殖凡纳滨对虾肠道和鳃的菌群结构分析[J].湖南农业大学学报（自然科学版）,2018,44(2):198-203,6.基金项目
上海市科技兴农重点攻关项目(沪农科攻字(2014)第3–4号) （沪农科攻字(2014）
上海市现代虾类产业技术体系专项(沪农科产字[2014]第5号) （沪农科产字[2014]第5号）
